While not specifically tested on gingival fibroblasts, this product is tested using mulitple mammalian cell lines. There is no reason to suspect that this product would not be suitable. For frozen cell stocks, the recommended maximum temperature is -70°C. Liquid nitrogen (-80°C) is a typical medium for this product. Please see the link below to review the product datasheet, which includes a brief protocol for freezing and thawing cells using this product:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/709/138/c6164pis.pdf
C6164
Cell Freezing Medium-DMSO 1×
sterile-filtered, suitable for cell culture
Synonym(s):
cryopreservation medium
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About This Item
NACRES:
NA.75
UNSPSC Code:
12352207
Pricing and availability is not currently available.
sterility
sterile-filtered
Quality Segment
form
liquid
technique(s)
cell culture | mammalian: suitable, cryopreservation: suitable
impurities
endotoxin, tested
shipped in
dry ice
storage temp.
−20°C
General description
Cell freezing or cryopreservation of cell culture is a routine process. It is essential to maintain the cellular integrity during storage. Majorly used cryoprotectants include glycerol, ethylene glycol and dimethyl sulfoxide(DMSO).
Application
Cell Freezing Medium-DMSO 1× has been used in the cryopreservation of human embryonic kidney HEK293 CNR2 cells, blood mononuclear cells and prostate cancer cells.
Other Notes
Complete ready-to-use medium. Proprietary formulation with Minimum Essential Medium, dimethyl sulfoxide, calf serum and fetal bovine serum.
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This Item | |||
|---|---|---|---|
| form liquid | form liquid | form liquid | form liquid |
| sterility sterile-filtered | sterility sterile-filtered | sterility - | sterility sterile-filtered |
| technique(s) cell culture | mammalian: suitable, cryopreservation: suitable | technique(s) cell culture | mammalian: suitable, cryopreservation: suitable | technique(s) cell culture | mammalian: suitable, cryopreservation: suitable | technique(s) cell culture | mammalian: suitable |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| shipped in dry ice | shipped in dry ice | shipped in wet ice | shipped in dry ice |
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Good day will the cell- freezing medium - DMSO be suitable for freezing of human gingival fibroblsts? can they be stored in -80 freezer or rather in liquid nitrogen with this freezer medium?
1 answer-
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When is the expiry date after bottle has been opened?
1 answer-
Following opening, the stability of the product is contingent upon end user handling. Shelf life will be maintained unless contaminants are introduced or the product is improperly stored or sealed. This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
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Does Product C6164, Cell Freezing Medium-DMSO, contain heat-inactivated serum?
1 answer-
No, the serum in this cell freezing media is not heat-inactivated.
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Is there insulin in Product C6164, Cell Freezing Medium-DMSO?
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No, there is no added insulin in this product. There may be traces of insulin from the serum used in this product.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Is it ok to sterile filter Product C6164, Cell Freezing Medium-DMSO?
1 answer-
Product No. C6164 is sterile-filtered. If the sterility of the product has been compromised, it can be re-filtered.
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How much DMSO is present in Product C6164, Cell Freezing Medium-DMSO?
1 answer-
The DMSO is at a 10% concentration.
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