Carbonic Anhydrase Isozyme II from bovine erythrocytes

Name: Carbonic Anhydrase Isozyme II from bovine erythrocytes
Brand: SIGMA

lyophilized powder, ≥3,000 W-A units/mg protein

Synonym(s):

Carbonate Dehydratase, Carbonate Hydrolyase, Carbonic Anhydrase II

Select a Size

Change View

About This Item

CAS Number:

9001-03-0

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-576-6

MDL number:

MFCD00081467

EC Number:

4.2.1.1 (BRENDA, IUBMB)

Specific activity:

≥3,000 W-A units/mg protein

Biological source:

bovine erythrocytes

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

biological source

bovine erythrocytes

Quality Segment

300

form

lyophilized powder

specific activity

≥3,000 W-A units/mg protein

mol wt

30 kDa

pI

~5.9

storage temp.

−20°C

Description

Application

Carbonic anhydrase from sigma has been used for the analysis of thermodynamic stability of the enzyme by measuring the binding affinity and stoichiometry. The enzyme has also been used to generate CD₄⁺ T cell lines specific for carbonic anhydrase during the study of autoimmune pancreatitis in rat.

Biochem/physiol Actions

Carbonic anhydrase is a zinc metalloenzyme that has a molecular weight of approximately 30,000 Da. The enzyme catalyzes the hydration of carbon dioxide to carbonic acid. It is involved in many physiological and pathological processes such as pH and CO₂ homeostasis, transport of bicarbonate and CO₂, biosynthetic reactions, bone resorption, calcification, and tumorigenicity. Therefore, inhibitors that block this enzyme can have therapeutic implications in glaucoma, epilepsy and Parkinson′s disease.

Other Notes

One Wilbur-Anderson (W-A) unit will cause the pH of a 0.02 M Trizma buffer to drop from 8.3 to 6.3 per min at 0 °C. (One W-A unit is essentially equivalent to one Roughton-Booth unit.)