The activity of this product is not determined. The material is prepared as a 10 mg/mL solution. However, historical data indicates the activity of the Bleomycin used to prepare this product may range from 1.5 - 2.0 units per milligram.
B7216
Bleomycin ready made solution
Cell culture tested, 10 mg/mL in H₂O
Synonym(s):
BLM, BLM A2, Blenoxane, Bleo, Bleomycin, Bleomycin A2, Blexane
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Size/SKU | Availability | Price |
|---|
About This Item
UNSPSC Code:
12352207
NACRES:
NA.76
Pricing and availability is not currently available.
Product Name
Bleomycin ready made solution, Cell culture tested, 10 mg/mL in H2O
Quality Segment
form
liquid
storage condition
protect from light
concentration
10 mg/mL in H2O
storage temp.
−20°C
General description
Bleomycin (BLM) is a glycopeptide antibiotic consist of 2 main isomers, BLM A2 and B2, obtained from Streptomyces verticillus. The main clinical application of Bleomycin is cancer treatment. Bleomycin causes oxidative degradation of RNA affecting G2 and M cell cycle phases. The anti-cancer mechanism of Bleomycin is mediated through iron chelation and generation of oxygen free radicals, leading to DNA cleavage and ultimately to cell death. Bleomycin hydrolase, the enzyme responsible for Bleomycin metabolism, is normally present at low levels in the lungs. Therefore, Bleomycin treatment may result in its accumulation in the lungs and can potentially cause lung injury due to lung cell death. The lung injury occurs through overproduction of reactive oxygen species causing pulmonary toxicity leading to fibrosis. The lung fibrosis by Bleomycin can be utilized for screening of antifibrotic agents.
Bleomycin has several important applications:
Bleomycin suggested concentration range for cell selection is 10-100μg/mL. Hence, it is recommended to dilute the ready made solution 1:100-1,000 in media of choice.
Bleomycin has several important applications:
- Bleomycin has been used to induce chronic lung disease in animal models as part of a research to find antifibrotic therapy for COVID-19 (SARS-CoV-2).
- Bleomycin - induced pulmonary fibrosis in-vivo experiments performed in mice by dose administration of 0.25-2mg/Kg.
- Bleomycin serves as a selective agent for gram-negative and gram-positive bacteria. Bleomycin cause DNA cleavage through formation of 1:1 complex with BLE protein. The resistance is obtained by encoding the ble gene of BLE protein from Streptoalloteichus hindustanus which prevents DNA cleavage.
- Bleomycin can be used for selection in different types of cell lines.
Bleomycin suggested concentration range for cell selection is 10-100μg/mL. Hence, it is recommended to dilute the ready made solution 1:100-1,000 in media of choice.
1 of 1
This Item | |||
|---|---|---|---|
| description Cell culture tested, 10 mg/mL in H2O | description 0.2 μm filtered, 20mg/mL in 20mM HEPES | description 10 mg/mL in DMSO, ≥98% (HPLC), Cell culture tested | description crystalline, 1.5-2.0 U/mg |
| Quality Level 200 | Quality Level 100 | Quality Level 200 | Quality Level 200 |
| form liquid | form liquid | form liquid | form crystalline |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| concentration 10 mg/mL in H2O | concentration - | concentration 10 mg/mL in DMSO | concentration - |
| storage condition protect from light | storage condition protect from light | storage condition protect from light | storage condition - |
signalword
Danger
hcodes
Hazard Classifications
Muta. 1B
Storage Class
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
wgk
WGK 2
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
-
What is the specific activity (U/mg) of the belomycin?
1 answer-
Helpful?
-