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B6125

Sigma-Aldrich

N-Benzoyl-L-tyrosine ethyl ester

Synonym(s):

BTEE

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About This Item

Linear Formula:
4-(HO)C6H4CH2CH(NHCOC6H5)CO2C2H5
CAS Number:
3483-82-7
Molecular Weight:
313.35
Beilstein:
2223819
EC Number:
222-469-2
MDL number:
MFCD00002388
UNSPSC Code:
12352209
eCl@ss:
32160406
PubChem Substance ID:
24891896
NACRES:
NA.25

form

powder

mp

118-121 °C (lit.)

storage temp.

−20°C

SMILES string

CCOC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)c2ccccc2

InChI

1S/C18H19NO4/c1-2-23-18(22)16(12-13-8-10-15(20)11-9-13)19-17(21)14-6-4-3-5-7-14/h3-11,16,20H,2,12H2,1H3,(H,19,21)/t16-/m0/s1

InChI key

SRLROPAFMUDDRC-INIZCTEOSA-N

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General description

N-Benzoyl-L-tyrosine ethyl ester (BTEE) is a substrate which is completely resistant to trypsin and is required for the assay of chymotrypsin.

Application

N-Benzoyl-L-tyrosine ethyl ester has been used for measuring chymotrypsin activity.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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C J Lo et al.
The Journal of surgical research, 69(2), 408-412 (1997-05-01)
Nuclear factor kappa B (NF kappa B) is an important transcriptional activator protein and is a crucial component of the host's response to infection. The activation of NF kappa B is correlated with the phosphorylation of inhibitory kappa B (I
Jason D Gillman et al.
Journal of agricultural and food chemistry, 63(5), 1352-1359 (2015-01-23)
Soybean seed contains antinutritional compounds that inactivate digestive proteases, principally corresponding to two families: Kunitz trypsin inhibitors (KTi) and Bowman-Birk inhibitors (BBI). High levels of raw soybean/soybean meal in feed mixtures can cause poor weight gain and pancreatic abnormalities via
K Y Jiang et al.
Biochimica et biophysica acta, 1433(1-2), 198-209 (1999-08-14)
Chemical glycosylation of bovine alpha-chymotrypsin, by a glucosamine adduct on the carboxyl group, results in the modification of its catalytic activity. The structural alterations of alpha-chymotrypsin resulting from its glycosylation are studied by immobilized metal-ion affinity chromatography (IMAC) and immobilized
R Chatterjee et al.
Photochemistry and photobiology, 51(1), 91-97 (1990-01-01)
Skh:HR-1 hairless mice were irradiated chronically with sub-erythemal doses of UVB radiation, and a number of biochemical parameters in the skin were determined after 6, 12, 18, and 24 wk of exposure. The parameters measured were water, collagen, elastin, and
J Kantorski et al.
Journal of bioluminescence and chemiluminescence, 7(1), 37-45 (1992-01-01)
We have studied an indirect role of serine and thiol proteases in the activation of human neutrophils in vitro. Stimulation was evaluated using a chemiluminescence (CL) generation system. Receptor-dependent and receptor-independent stimuli were studied, e.g. opsonized zymosan, formyl-methionyl-leucyl-phenylalanine, platelet activating
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Articles

Chymotrypsin

Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.

Protocols

Procedure for Enzymatic Assay of α-Chymotrypsin (EC 3.4.21.1)

Follow our procedure for the determination of a chymotrypsin activity. This enzymatic assay of alpha chymotrypsin guides you through the entire process and necessary calculations.

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