This product has not been tested for labeling bacteria internally, including Staphylococcus aureus. Therefore, no data is available for this application. A literature search is recommended for specific applications.
46950
Fluorescein 5(6)-isothiocyanate
BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)
Fluorescein 5(6)-isothiocyanate
Synonym(s):
FITC, Fluorescein isothiocyanate
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Size/SKU | Availability | Price |
|---|
About This Item
Empirical Formula (Hill Notation):
C21H11NO5S
CAS Number:
Molecular Weight:
389.38
UNSPSC Code:
12171500
NACRES:
NA.32
PubChem Substance ID:
EC Number:
248-207-7
Beilstein/REAXYS Number:
1407295
MDL number:
Pricing and availability is not currently available.
Quality Segment
product line
BioReagent
assay
≥90% (HPLC)
form
powder
mol wt
389.38 g/mol
color
orange to dark orange, yellow to dark yellow
solubility
acetone: 0.1 g/10 mL, clear to very slightly hazy
fluorescence
λex 492 nm; λem 518 nm in 0.1 M phosphate pH 8.0
suitability
suitable for fluorescence
storage temp.
−20°C
SMILES string
Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
InChI
1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
InChI key
MHMNJMPURVTYEJ-UHFFFAOYSA-N
General description
Fluorescein 5(6)-isothiocyanate, also known as FITC, is a weakly acidic hydroxyxanthene whose overall size is small, with both carboxylic acid and isothiocyanate substituents on the pendant phenyl ring. The dye has a moderately sized, nonplanar aromatic system. The isothiocyanate group reacts with nucleophilic substituents (e.g. amino, hydroxyl, thiol) of biomolecules to be attached as a fluorescent label. The maximum emission of the dye is at 519nm in the same solvent.
Application
Fluorescein 5(6)-isothiocyanate (FITC) labelled molecules are used in non-microscopic immunoassays and physiological tracing experiments. FITC is widely used in flow cytometry experiments for staining proteins, nuclei, and subnuclear particles. White blood cells (WBCs) are marked with FITC to study their response toward a chemoattractant, eventually being measured.
For fluorescent labeling of proteins (e.g. immunofluorescence); H Protein labeling for gel chromatography
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
1 of 1
This Item | |||
|---|---|---|---|
| suitability suitable for fluorescence | suitability - | suitability - | suitability - |
| fluorescence λex 492 nm; λem 518 nm in 0.1 M phosphate pH 8.0 | fluorescence - | fluorescence λex 492 nm; λem 518 nm (green) | fluorescence λex 492 nm; λem 518 nm (green) |
| color orange to dark orange, yellow to dark yellow | color yellow to orange | color orange to dark orange | color yellow to very dark yellow |
| form powder | form powder | form powder | form powder |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
Still not finding the right product?
Explore all of our products under Fluorescein 5(6)-isothiocyanate
Storage Class
11 - Combustible Solids
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Skin Irrit. 2 - Skin Sens. 1B
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
-
excuse me， can the product of Fluorescein 5(6)-isothiocyanate be used to lable bacteria，such as Staphylococcus aureus？ thanks
1 answer-
Helpful?
-
-
Is it suitable for fluorescence microscopy?
1 answer-
This product is tested for fluorescent labeling of proteins. While not tested specifically for use in fluorescent microscopy, this compound is routinely used in cell-based studies applying this technique.
Please see the link below to review the Peer Reviewed Publications:
https://www.sigmaaldrich.com/search/46950?focus=papers&page=1&perpage=30&sort=relevance&term=46950&type=citation_searchHelpful?
-