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19899
CHAPS 100 mM solution
Synonym(s):
CHAPS solution
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About This Item
Empirical Formula (Hill Notation):
C32H58N2O7S
CAS Number:
Molecular Weight:
614.88
NACRES:
NA.25
PubChem Substance ID:
UNSPSC Code:
12161902
MDL number:
Pricing and availability is not currently available.
description
zwitterionic
Quality Segment
form
liquid
mol wt
614.88 g/mol
concentration
100 mM in H2O
technique(s)
protein quantification: suitable
CMC
8 mM
transition temp
cloud point >100 °C
density
1.01 g/mL at 20 °C
storage temp.
2-8°C
SMILES string
C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)CC4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C
InChI
1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22?,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1
InChI key
UMCMPZBLKLEWAF-RFCNGIAKSA-N
General description
3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) is a biocompatible surfactant. It is a derivative of cholic acid comprising a hydrophobic cholesterol-like skeleton possessing three hydroxyl groups and a zwitterionic aliphatic tail.[1]
Application
Biochem/physiol Actions
3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) exhibits the properties of both sulfobetaine-type surfactants and bile salts. It is involved in the protein extraction process for proteomic analysis and reconstitution of membrane proteins into lipid vesicles (proteoliposomes). CHAPS is broadly used in biochemistry for membrane protein and receptor isolation and purification. It is conveniently used in pharmaceuticals and medicines due to its biocompatibility, noncharged character, nondenaturing, and disaggregating properties.[1]
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This Item | |||
|---|---|---|---|
| technique(s) protein quantification: suitable | technique(s) dialysis: suitable | technique(s) - | technique(s) - |
| form liquid | form crystals | form crystalline powder | form solid |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 10-30°C | storage temp. 2-8°C |
| density 1.01 g/mL at 20 °C | density - | density - | density - |
| CMC 8 mM | CMC 4 mM, 6 mM (20-25°C) | CMC 6 mM (20-25°C) | CMC 6 mM (20-25°C) |
| mol wt 614.88 g/mol | mol wt micellar avg mol wt 6150, 614.88 g/mol (anhydrous basis) | mol wt micellar avg mol wt 6150 | mol wt micellar avg mol wt 6150 |
Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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