06627

2-Aminoacridone

BioReagent, suitable for fluorescence, ≥98.0% (HPLC)

• Synonym(s): 2-Amino-9(10H)-acridinone, 2-amino-10H-acridin-9-one, AMAC
• Empirical Formula (Hill Notation): C₁₃H₁₀N₂O
• CAS Number: 27918-14-5
• Molecular Weight: 210.23
• NACRES: NA.32
• PubChem Substance ID: 329748253
• UNSPSC Code: 12352108
• MDL number: MFCD00037397
• Beilstein/REAXYS Number: 172520

Properties

• product line: BioReagent
• Quality Segment: 100
• assay: ≥98.0% (HPLC)
• form: powder
• mol wt: 210.23 g/mol
• concentration: ≤100%
• color: yellow to dark yellow
• solubility: DMF: soluble, DMSO: soluble
• fluorescence: λ_ex 420 nm; λ_em 542 nm in 0.1 M Tris pH 8.0
• suitability: suitable for fluorescence
• InChI: 1S/C13H10N2O/c14-8-5-6-12-10(7-8)13(16)9-3-1-2-4-11(9)15-12/h1-7H,14H2,(H,15,16)
• InChI key: PIGCSKVALLVWKU-UHFFFAOYSA-N

Description

Application

2-Aminoacridone is a highly fluorescent aromatic, which contains a primary amine group that reacts with an aldehyde group at the reducing end of a carbohydrate and is reduced to a stable amine derivative by sodium borohydride (NaBH₄). Picomolar levels of glycan compounds can be detected using this fluorophore. The resulting derivatzed compounds can be separated by reverse-phase HPLC and detected by positive-ion electrospray MS . An intense fluorescent, hydrophobic probe that is stable over a wide pH range is useful in the derivatization of glycans to allow for the analysis of complex oligosaccharides using micellar electrokinetic capillary chromatography and reverse- and normal-phase chromatography coupled with mass spectroscopy to determine relative concentrations and structural identity of individual oligosaccharides . The λ excitation and λ emission are 425 nm and 532 nm, respectively.

Fluorescent label for glycans and saccharides.

Fluorescent label for glycans and saccharides. The analysis of fluorophore-labeled glycans by high-resolution polyacrylamide gel electrophoresis.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H315,H319,H335
• pcodes: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves