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E4378

Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid

≥97.0%

Synonym(s):

EGTA, Egtazic acid, Ethylene-bis(oxyethylenenitrilo)tetraacetic acid, Glycol ether diamine tetraacetic acid

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About This Item

Linear Formula:
[-CH2OCH2CH2N(CH2CO2H)2]2
CAS Number:
Molecular Weight:
380.35
UNSPSC Code:
12352106
NACRES:
NA.77
PubChem Substance ID:
EC Number:
200-651-2
Beilstein/REAXYS Number:
1717370
MDL number:
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biological source

synthetic (organic)

Quality Segment

assay

≥97.0%

form

powder

reaction suitability

reagent type: chelator

loss

≤1.0% loss on drying

mp

241 °C (dec.) (lit.)

solubility

1 M NaOH: 1.1 g/10 mL, clear, colorless

storage temp.

room temp

SMILES string

OC(=O)CN(CCOCCOCCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C14H24N2O10/c17-11(18)7-15(8-12(19)20)1-3-25-5-6-26-4-2-16(9-13(21)22)10-14(23)24/h1-10H2,(H,17,18)(H,19,20)(H,21,22)(H,23,24)

InChI key

DEFVIWRASFVYLL-UHFFFAOYSA-N

General description

solubility in water increases with pH

Application

A chelating agent useful for the determination of calcium in the presence of magnesium.
Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid has been used as a component of:
  • ice-cold cardioplegia solution for transverse aortic constriction in rats
  • ice-cold lysis buffer for protein extraction and whole extract cells
  • buffer A, B and, C for the extraction of mitochondria from HEK293F cells

Biochem/physiol Actions

Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid A is a chelating agent used for the determination of calcium in the presence of magnesium.

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This Item
E03964100-OP03777
assay

≥97.0%

assay

≥97 .0%

assay

-

assay

≥99.0% (T)

Quality Level

400

Quality Level

400

Quality Level

100

Quality Level

300

form

powder

form

powder

form

crystalline powder (Fine)

form

powder

storage temp.

room temp

storage temp.

-

storage temp.

-

storage temp.

-

mp

241 °C (dec.) (lit.)

mp

241 °C (dec.) (lit.)

mp

-

mp

241 °C (dec.) (lit.)

solubility

1 M NaOH: 1.1 g/10 mL, clear, colorless

solubility

1 M NaOH: 0.1 M, clear, colorless, H2O: soluble (increases as pH is increased)

solubility

-

solubility

0.1 M NaOH: 0.1 M at 20 °C, clear, colorless


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Storage Class

11 - Combustible Solids

wgk

WGK 2

ppe

Eyeshields, Gloves, type N95 (US)



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Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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