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11363905910

Roche

Digoxigenin-11-ddUTP

solution, =85% (HPLC), pkg of 25 μL (25 nmol; 1mM)

Synonym(s):

digoxigenin

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About This Item

UNSPSC Code:
41116100

Quality Level

Assay

85% (HPLC)

form

solution

mol wt

(Molecular weight: Mr = 1074.7 (DIG-11-ddUTP-Li4))

packaging

pkg of 25 μL (25 nmol; 1mM)

manufacturer/tradename

Roche

storage temp.

−20°C

General description

Digoxigenin-11-ddUTP is a 1 mM solution of the tetralithium salt. Digoxigenin-11-ddUTP is a component of probe used for end labeling. Digoxigenin-11-ddUTP is an alternative to classical radioactive labeling for electrophoretic mobility shift assay and is highly stable compared to radioactive probes.

Application

Digoxigenin-11-ddUTP has been used:
  • for labeling of DNA fragments in linker histone binding assay
  • for end labeling of NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) for electrophoretic motility shift assay and labelling of ATP citrate lyase gene for gel shift assay

for end labeling of DNA for fragmentation reactions

Quality

Typical analysis: 85% DIG-11-ddUTP (HPLC, area%)
Function tetsted by end labeling.

Formula: C43H61N4O20P3Li4

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

does not flash

Flash Point(C)

does not flash


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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M Martínez-Bueno et al.
Journal of bacteriology, 176(20), 6334-6339 (1994-10-01)
The structural gene of the enterococcal peptide antibiotic AS-48 (as-48) has been identified and cloned by using two degenerate 17-mer DNA oligonucleotides on the basis of the amino acid sequences of two peptides obtained by digestion of the antibiotic with
Transcriptional regulation of the ATP citrate-lyase gene by sterol regulatory element-binding proteins
Sato R, et al.
The Journal of Biological Chemistry, 275(17), 12497-12502 (2000)
Inhibition of extracellular signal-regulated kinases 1/2 provides neuroprotection in spinal cord ischemia/reperfusion injury in rats: relationship with the nuclear factor-kappaB-regulated anti-apoptotic mechanisms
Lu K, et al.
Journal of Neurochemistry, 114(1), 237-246 (2010)
HILS1 is a spermatid-specific linker histone H1-like protein implicated in chromatin remodeling during mammalian spermiogenesis
Yan W, et al.
Proceedings of the National Academy of Sciences of the USA, 100(18), 10546-10551 (2003)
Non-radioactive electrophoretic mobility shift assay using digoxigenin-ddUTP labeled probes
Kass J, et al.
Drosophila Information Service, 83, 185-188 (2000)

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