11074547001

Insulin-Transferrin-Sodium Selenite Supplement

suitable for cell culture, lyophilized, pkg of 50 mg (for 5 l medium)

• Synonym(s): ITS
• UNSPSC Code: 12352207

Properties

• biological source: human serum (transferrin); synthetic (sodium selenite)
• Quality Level: 100
• recombinant: expressed in human cells
• sterility: non-sterile; 0.2 μm filtered
• Assay: >98% (Transferrin, SDS-PAGE); >99% (Insulin, HPLC); >99% (Sodium selenite)
• form: lyophilized
• packaging: pkg of 50 mg (for 5 l medium)
• manufacturer/tradename: Roche
• technique(s): cell culture | mammalian: suitable
• solubility: water: miscible
• UniProt accession no.: P01317; P02786
• storage temp.: 2-8°C
• Gene Information: bovine ... INS(280829) ; human ... TFRC(7037)

Description

General description

The Insulin-Transferrin-Sodium Selenite Supplement contains the most essential growth-promoting components of serum-free culture media in an optimized ratio.
Source: Insulin- bovine pancreas; transferrin- human serum; Sodium selenite-synthetic

The insulin-transferrin-sodium selenite (ITS) supplement comprises three of the most crucial growth factors for many cell types. Insulin is a component of serum-free media formulations for all primary cells and cell lines. It stimulates cell growth, increases fatty acid and glycogen synthesis in a serum-free medium. Transferrin is an essential growth factor for many cell types. Selenium is very often necessary for optimal cell growth. ITS permits an appreciable reduction in serum requirements for cellular growth. ITS is also an antioxidant that supports the growth of in vivo derived (IVD) embryos. Insulin promotes embryonic growth and metabolism. The iron transport in the embryo is mediated by transferrin. Sodium selenite protects from oxidative damage and inhibits lipid peroxidation. ITS is useful in human chondrocytes monolayer culture.

Application

The Insulin-Transferrin-Sodium Selenite Supplement is used in the cell culture media as a basal growth-factor supplement to which other cell type-specific nutrients and growth factors are added.

Preparation Note

Working concentration: Insulin, 5 μg/ml, transferrin, 5 μg/ml, sodium selenite, 5 ng/ml (3.0 x 10^-8 M)

Storage conditions (working solution): -15 to -25 °C.
Prepare appropriate aliquots and avoid repeated freezing and thawing.

Reconstitution

The insulin-transferrin-sodium selenite supplement should be reconstituted in 5 ml or 25 ml sterile double-dist. water (1000 x concentrated stock solution).
Further dilution with culture medium.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): does not flash
• Flash Point(C): does not flash