MABT1550

Anti-Lactadherin/MFGE8 Antibody, clone 5C5

• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 5C5, monoclonal
• mol wt: calculated mol wt 45.73 kDa; observed mol wt ~47 kDa
• purified by: using protein G
• species reactivity: human, boar
• packaging: antibody small pack of 100 μL
• technique(s): immunofluorescence: suitable; western blot: suitable
• isotype: IgG1κ
• epitope sequence: Unknown
• UniProt accession no.: P79385
• shipped in: 2-8°C
• target post-translational modification: unmodified
• Gene Information: pig ... MFGE8(397545)

Description

General description

Lactadherin (UniProt: P79385; also known as MFGM, Milk fat globule-EGF factor 8, MFG-E8, SED1, Sperm surface protein SP47, PP47) is encoded by the MFGE8 gene (Gene ID: 397545) in Boar. MFG-E8 is a peripheral membrane glycoprotein that is widely expressed in many cell types. At its N-terminal region, it contains two EGF-like repeats (aa 2-41 and 44-88). The second EGF repeat contains an RGF integrin-binding domain that allows its interaction with and activation of Vβ3 and Vβ5 integrins. It also has two C-terminal F5/8 type C domains. The second F5/8 type C domain exhibits high affinity for binding to phosphatidylserine present in cell membranes. MFG-E8 displays high affinity for glycoproteins on egg surface and contributes to the ability of sperm to adhere to the egg in an integrin-independent manner. It has been reported that although MFG-E8 is not absolutely essential for fertilization, its absence is shown to reduce fertility in mice. It has also been implicated in a variety of other physiological functions. MFG-E8 can bind apoptotic cells via its F5/8C domains, and its RGD motif is shown to be essential for cell engulfment by macrophages. In the mammary glands, it is required for clearance of apoptotic cells during mammary gland involution at the end of lactation. Defects in or lack of MFG-E8 can cause the accumulation of apoptotic cell remnants, duct distension and mastitis. (Ref.: Zigo, M., et al. (2015). Cell Tissue Res. 359(3); 895-908; Ensslin, MA., and Shur, BD. (2007). Proc. Natl. Acad. Sci. USA 104(8); 2715-2720).

Specificity

Clone 5C5 is a mouse monoclonal antibody that detects Sperm surface protein (Lactadherin).

Immunogen

Purified Sperm surface protein from Boar.

Application

Quality Control Testing

Evaluated by Western Blotting in MDA-MB-231 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Lactadherin/MFGE8 in MDA-MB-231 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:500 dilution from a representative lot detected Lactadherin/MFGE8 in HeLa cell lysate.

Western Blotting Analysis: A representative lot detected Lactadherin/MFGE8 in Western Blotting applications (Zigo, M., et al. (2015). Cell Tissue Res. 359(3):895-908).

Immunofluorescence Analysis: A representative lot detected Lactadherin/MFGE8 in Immunofluorescence applications (Zigo, M., et al. (2015). Cell Tissue Res. 359(3):895-908).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Lactadherin/MFGE8 , clone 5C5, Cat. No. MABT1550, is a mouse monoclonal antibody that detects Lactadherin and is tested for use in Immunofluorescence and Western Blotting.

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1