MAB5352

Anti-Notch 1 Antibody, clone mN1A

clone mN1A, Chemicon^®, from mouse

• Synonym(s): Anti-AOS5, Anti-AOVD1, Anti-TAN1, Anti-hN1
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: mN1A, monoclonal
• Application: ICC, IHC, IP, WB
• Citations: 12

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: mN1A, monoclonal
• species reactivity: rat, mouse, feline
• packaging: antibody small pack of 25 μg
• manufacturer/tradename: Chemicon^®
• technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG1
• NCBI accession no.: NM_017617.3
• UniProt accession no.: P46531
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... NOTCH1(4851)

Description

General description

272.5 kDa (also 230 kDa processed form)

Notch1 consists of three domains. The extracellular domain containing 36 epidermal growth factor (EGF) repeats and 3 lin-12/Notch repeats (LNR) and functions as a receptor in cell-cell interactions. There is a single transmembrane domain (TM). The intracellular domain contains six cdc10/ankyrin repeats (cdc10) and the newly defined Notch cytokine response (NCR) region, which contains a putative bipartite NLS (nuclear localization signal).

Immunogen

Cdc10-NCR region of mouse Notch1 expressed as a GST fusion protein.

Application

Anti-Notch 1 Antibody, clone mN1A is a high quality Mouse Monoclonal Antibody for the detection of Notch 1 & has been validated in ICC, IHC, IP & WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Western blot using ECL

Immunohistochemistry

Immunocytochemistry

Immunoprecipitation

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Recognizes Notch1, intracellular domain. The antibody has a high affinity for the "activated" intracellular form of Notch1, but a low affinity for the full-length (unprocessed or heterodimeric cell surface) forms. No reactivity with Notch2, 3 or 4.

Physical form

Format: Purified

Protein A purified

Purified immunoglobulin. Liquid.

Preparation Note

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
In fetal tissues, use spleen, brain stem and lung. In most adult tissues, use lymphoid tissues.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 04-1046

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 2