70923
BugBuster® (primary amine-free) Extraction Reagent
Synonym(s):
Protein Extraction Reagent
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About This Item
NACRES:
NA.77
UNSPSC Code:
41106511
Pricing and availability is not currently available.
form
liquid
Quality Segment
manufacturer/tradename
Novagen®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
input
bacteria and yeast sample(s)
shipped in
ambient
storage temp.
10-30°C
General description
BugBuster (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilization or cross-linking. The PIPPS buffer used in BugBuster (primary amine-free) has a similar buffer capacity and pH range as the original Tris-buffered BugBuster, but will not complex metal ions, also making it ideally suited for extraction of metal dependent proteins.
Features and Benefits
Primary Amine-Free Formulation: BugBuster® (primary amine-free) Extraction Reagent is specially designed for applications where primary amines would interfere with protein extract. This means that academic researchers can purify metal-dependent proteins or proteins to be used for immobilization or crosslinking without worrying about the presence of primary amines, which could cause interference.
Enhanced Extraction: The extraction process can be further enhanced by the addition of rLysozyme Solution and Benzonase Nuclease, which helps to efficiently extract and purify proteins from the sample.
Metal Ion Compatibility: The PIPPS buffer used in the primary amine-free formulation of BugBuster® has a similar buffer capacity and pH range as the original Tris-buffered BugBuster® but will not complex metal ions. This makes it an ideal choice for extraction of metal-dependent proteins
Enhanced Extraction: The extraction process can be further enhanced by the addition of rLysozyme Solution and Benzonase Nuclease, which helps to efficiently extract and purify proteins from the sample.
Metal Ion Compatibility: The PIPPS buffer used in the primary amine-free formulation of BugBuster® has a similar buffer capacity and pH range as the original Tris-buffered BugBuster® but will not complex metal ions. This makes it an ideal choice for extraction of metal-dependent proteins
Legal Information
BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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This Item | |||
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| description - | description - | description Novagen′s PhosphoSafe Extraction Buffer efficiently extracts cytosolic proteins from mammalian and insect cells while preserving their phosphorylation state. | description - |
| form liquid | form liquid | form liquid | form solid |
| storage temp. 10-30°C | storage temp. 2-8°C | storage temp. −70°C | storage temp. - |
| Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
| input bacteria and yeast sample(s) | input bacteria and yeast sample(s) | input general use sample(s) | input general use sample(s) |
| shipped in ambient | shipped in wet ice | shipped in dry ice | shipped in - |
| manufacturer/tradename Novagen® | manufacturer/tradename Novagen® | manufacturer/tradename Novagen® | manufacturer/tradename - |
Storage Class
10 - Combustible liquids
wgk
WGK 2
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