SAE0066

Adenylyl Cyclase Toxin from Bordetella pertussis

• UNSPSC Code: 12352200
• NACRES: NA.26

Properties

• biological source: Bordetella pertussis Tohama I
• Quality Level: 200
• Assay: ≥70%
• form: liquid
• specific activity: ≥ 50 units/mg protein
• technique(s): cell culture | mammalian: suitable
• suitability: suitable for molecular biology
• application(s): detection
• storage temp.: −20°C
• Gene Information: Bordetella pertussis Tohama I ... CyaA(69600712)

Description

General description

Research area: IMMUNO AND CKS

Adenylate Cyclase Toxin (ACT or CyaA) is a member of the extensive family of toxins known as Repeat in Toxin (RTX), which are produced by Gram-negative organisms. ACT is encoded by the cyaA gene and secreted extracellularly in the form of a soluble protein. It exhibits both adenylate cyclase enzymatic activity and hemolytic activity. The synthesis, maturation, and secretion of ACT are regulated by the CyaCABD operon. Moreover, its specific cellular receptor, CD11b/CD18 integrin (αMβ2, Mac-1, or CR3), is expressed on myeloid phagocytes.

Application

Adenylyl Cyclase Toxin from Bordetella pertussis has been used as Gα(i/o) inhibitor to study the involvement of the sphingosine 1-phosphate receptor 2/Gα(12/13)/MAPK signaling pathway in the priming and activation of NLRP3 inflammasome during cholestatic liver injury.

Biochem/physiol Actions

Adenylate Cyclase Toxin (CyaA) is responsible for inhibiting the phagocytic activities of neutrophils and macrophages by impairing oxidative response and chemotaxis, ultimately leading to cell apoptosis or necrosis. Additionally, ACT can upregulate the expression of MHC class II and costimulatory molecules on dendritic cells, thereby reducing proinflammatory cytokine production.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable