L8275
Lectin from Galanthus nivalis (snowdrop)
lyophilized powder
Synonym(s):
Galanthus nivalis lectin, GNL
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About This Item
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form
lyophilized powder
Quality Level
potency
<20 μg per mL agglutination activity
composition
Protein, ≥90% Lowry
impurities
salt, essentially free
storage temp.
2-8°C
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General description
Lectin from Galanthus nivalis (snowdrop) is a tetrameric, 12 kDa mannose binding lectin. It belongs to the family, Amaryllidacea. It is specific to α-1,3- or 1,6- linked D-mannose of carbohydrates. Snowdrop lectins are toxic to insects.
Application
Lectin from Galanthus nivalis (snowdrop) has been used:
- to coat ELISA plates
- as reverse transcriptase inhibitor
- in ELISA for detection of envelope glycoproteins of the human and simian immunodeficiency viruses
Biochem/physiol Actions
Lectin from Galanthus nivalis (snowdrop) provide resistance against pests and are used in transgenic plants. Snowdrop lectin functions as a carrier to transport fused proteins to the hemolymph as it crosses gut epithelium of target species to exhibit insecticidal effects.
GNL agglutinates rabbit erythrocytes; human erythrocytes are not agglutinated. GNL sugar specificity is directed to the nonreducing end of the terminal α-D-mannosyl residue of glycoconjugates.
Preparation Note
Purified by affinity chromatography according to Kaku.
Analysis Note
Agglutination activity is expressed in μg/ml and is determined from serial dilution in phosphate buffered saline, pH 7.2 of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of rabbit erythrocytes after 1 hr incubation at 25 °C.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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