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Key Documents

H9034

Sigma-Aldrich

Hepatitis C virus core antigen

~1 mg/mL, ≥95% (SDS-PAGE), recombinant, expressed in E. coli

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About This Item

MDL number:
UNSPSC Code:
12352202
NACRES:
NA.32

recombinant

expressed in E. coli

Quality Level

Assay

≥95% (SDS-PAGE)

mol wt

22 kDa (plus 114 kDa galactosidse)

concentration

~1 mg/mL

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

hepatitis C virus ... HCVgp1(951475)
human ... HBVgp4(944568)

General description

Hepatitis C virus core antigen (HCV-cAg) is a well-preserved antigenic protein of the internal capsid.
Hepatitis C virus glycoprotein 1 (HCVgp1), also referred to as polyprotein, is crucial for the induction of double-membrane vesicles, sites of HCV RNA amplification. The amino terminal of polyprotein contains the structural proteins core, envelope protein 1 (E1) and E2. The proteolytic cleavage of polyprotein results in the formation of 10 mature viral proteins which are needed for viral replication and assembly.
amino acids 2-192 of HCV polyprotein

Specificity

Reacts strongly with human HCV positive serum.

Application

Hepatitis core antigen (HCA) has been used as an analyte in enzyme-linked immunosorbent assay (ELISA) to study the mechanism of bovine serum albumin (BSA) blocking in common ELISA protocols.

Biochem/physiol Actions

Hepatitis C virus core antigen (HCV-cAg) may be a less expensive way to diagnose active hepatitis C virus (HCV) infection in the hemodialysis (HD) population.
Positive control for HCV antibodies.

Other Notes

HCV core antigen [2-192]-Galactosidase-tagged at the N-terminus.

Physical form

Solution in 8 M Urea, 20 mM Tris-HCl, pH 8.0, 10 mM 2-mercaptoethanol.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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