Skip to Content
Merck
All Photos(1)

Documents

GALS

Sigma-Aldrich

β-Galactosidase Reporter Gene Staining Kit

Synonym(s):

β-galactosidase, LacZ

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12161503
NACRES:
NA.32

usage

 kit sufficient for 100 tests (using a 3.5 cm dish)

Quality Level

technique(s)

microbe id | staining: suitable

shipped in

dry ice

storage temp.

−20°C

General description

β-galactosidase (β-gal) catalyzes the conversion of X-gal to galactose and 5-bromo-4-chloro-3-hydroxyindole. The functionality of β-gal is indirectly measured by the blue precipitate of dimeric 5-bromo-4-chloro-3-hydroxyindole in the presence of potassium ferricyanide or ferrocyanide. The colorimetric detection methods also use other substrates of β-gal like O-nitrophenyl-β-D-galactopyranoside (ONPG). β-gal is a cost-effective, simple and standard reporter system. The Galactosidase Reproter Gene Staining Kit provides a method for determining the percentage of cells transfected with the plasmid expressing the β-galactosidase gene, LacZ. It can also be used to visualize the specific expression of the reporter gene in tissue sections by microscopy.

Application

β-Galactosidase Reporter Gene Staining Kit has been used for histochemical staining of animal cells or tissues such as:
  • sagittal brain sections in histoenzymology
  • pancreas or colon sections in β-galactosidase reporter gene assay
  • mice E10.5 -11.5 embryo and embryo sections

Other Notes

The substrate used in this kit is X-Gal (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside) which results in an indigo blue-colored staining of transfected cells and tissues. This test provides a method for determining the percentage of transfected cells that are expressing LacZ or for microscopically visualizing reporter gene expression in tissue sections.

related product

Product No.
Description
Pricing

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

Supplementary Hazards

Storage Class Code

3 - Flammable liquids

Flash Point(F)

132.8 °F

Flash Point(C)

56 °C


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Maria Boichuck et al.
Aging, 11(9), 2889-2897 (2019-05-16)
Here, we reported for the first time an increased expression of c-Met protein in primary cultures of human dermal and pulmonary fibroblasts of late passages. This suggests that c-Met could serve as an early marker of cellular senescence (CS). The
D Long et al.
Journal of virology, 68(9), 5890-5898 (1994-09-01)
Human immunodeficiency virus type 1 (HIV-1) infects some cell types which lack CD4, demonstrating that one or more alternative viral receptors exist. One such receptor is galactosylceramide (GalCer), a glycosphingolipid distributed widely in the nervous system and in colonic epithelial
Snir Yehuda et al.
Biogerontology, 18(4), 525-533 (2017-03-03)
The role of telomere shortening in the induction of replicative cellular senescence (CS) is well known and as a result, the involvement of telomerase and in particular its catalytic subunit, the telomerase reverse transcriptase (TERT) in CS has also been
Wendy Phillips et al.
Methods in molecular biology (Clifton, N.J.), 549, 137-155 (2009-04-21)
Animal models of neurodegenerative disease are excellent tools for studying pathogenesis and therapies including cellular transplantation. In this chapter, we describe different models of Huntington's disease and Parkinson's disease, stereotactic surgery (used in creation of lesion models and transplantation) and
beta-Galactosidase assay
Smale ST
Cold Spring Harbor Protocols, 2010, pdb-prot5423 (2010)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service