G1546

Anti-Green Fluorescent Protein (GFP) antibody, Mouse monoclonal

clone GSN149, purified from hybridoma cell culture

• Synonym(s): Mouse Anti-GFP
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: GSN149, monoclonal
• form: buffered aqueous solution
• packaging: antibody small pack of 25 μL
• technique(s): indirect ELISA: suitable; western blot: 1-2 μg/mL using extracts of cells expressing GFP fusion proteins
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Monoclonal Anti-Green Fluorescent Protein (GFP) (mouse IgG1 isotype) is derived from the hybridoma GSN149 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide of the Green fluorescent protein from jellyfish Aequorea victoria. GFP is a 27 kDa protein (238 amino acids) that can produce a strong green fluorescence without the need for a substrate. It absorbs light maximally at 395 nm and emits a bright green fluorescence with a peak at 509 nm. The GFP chromophore is formed through cyclization and oxidation of an internal tripeptide motif (Ser⁶⁵, Gly⁶⁹ and Tyr⁶⁶).

The antibody reacts specifically with GFP-fusion proteins.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.

• Immunocytochemistry
• Immunofluorescence-cell culture cells
• Immunoprecipitation
• Western Blotting
• Western blotting following immunoprecipitation

Monoclonal Anti-Green Fluorescent Protein (GFP) antibody produced in mouse was used in surface plasmon resonance (SPR) gold chip analysis to study the cysteine- and domain-mediated immobilization of enhanced GFP model protein.

Biochem/physiol Actions

GFP is implicated in protein detection and localization in living cells, as well as gene expression monitoring in prokaryotes and eukaryotes. It detects protein-protein interactions in living cells.

Green fluorescent protein, produced in the jellyfish, Aequorea victoria, acts as secondary fluorescent protein in an energy transfer reaction to produce green light. It ability to produce a highly visible, internal fluorophore is widely applicable as marker for gene expression and to produce chimeric proteins to understand protein biochemistry.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves