Products 69451-M and 70236-M are exactly the same competent cells. The only difference is in the packaging. The 70236 is already in 50uL aliquots, which are single-use, each aliquot is used for one reaction. This reference is available in two sizes of 11 or 22 reactions. While product 69451 is available in single 0.4 or 1mL volumes which may be thawed once and dispensed to smaller aliquots.
The difference between the 69451-M/70236-M products and the CMC0015 BL21 is the type of plasmid that they express for the T7 lysozymes. The expression of T7 lysozyme reduces the basal expression of the target genes, inhibiting the T7 RNA polymerase. Specifically, with the expression of the plasmid pLysE (Ref. CMC0015) a higher expression of the levels of T7 Lysozime is obtained in comparison with the plasmid pLysS (ref. 69451-M/70236-M). Consequently, with reference CMC0015 there is even less risk of basal expression of the target protein in the absence of IPTG stimulation. This could be particularly relevant for toxic proteins that could affect competent cell viability.
CMC0015
BL21(DE3) pLysE Chemically Competent Cells
Escherichia coli, rod shaped
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Product Name
BL21(DE3) pLysE Chemically Competent Cells, for protein expression
biological source
Escherichia coli
grade
for molecular biology
growth mode
adherent or suspension
morphology
rod shaped
technique(s)
microbiological culture: suitable
cell transformation
competent cell type: chemically competent
transformation efficiency: ≥1 x 107 cfu/μg
shipped in
dry ice
storage temp.
−70°C
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General description
These cells have a higher level of repression by T7 lysozyme, offering the greatest control over expression of inserts.
Genotype
F – ompT hsdSB (rB- mB-) gal dcm (DE3)
Features and Benefits
- a transformation efficiency of >1 × 107 cfu/μg.
- economical prices
- offering superior value for everyday protein expression work.
Components
- BL21(DE3) pLysE chemically competent cells
- pUC 19 transformation control DNA
- recovery medium for expression
related product
Storage Class Code
10 - Combustible liquids
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BL21 Chemically Competent Cells come in aliquots for easy transformation with heat shock method, facilitating molecular biology experiments.
BL21 Chemically Competent Cells come in aliquots for easy transformation with heat shock method, facilitating molecular biology experiments.
BL21 Chemically Competent Cells come in aliquots for easy transformation with heat shock method, facilitating molecular biology experiments.
BL21 Chemically Competent Cells come in aliquots for easy transformation with heat shock method, facilitating molecular biology experiments.
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What is the difference between the strain expressing pLysE and pLysS?
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