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A1603

Sigma-Aldrich

Alginate Lyase

powder, ≥10,000 units/g solid

Synonym(s):

poly-beta-D-mannuronate lyase

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About This Item

CAS Number:
Enzyme Commission number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

form

powder

Quality Level

specific activity

≥10,000 units/g solid

storage temp.

2-8°C

General description

Alginate lyases is present in several marine algae, marine mollusks and a vast range of marine and terrestrial bacteria.

Application

Alginate Lyase has been used:
  • in the pre-processing of enzyme
  • to de-encapsulate cells
  • to digest Pseudomonas exopolysaccharide to facilitate electroporation

Alginate Lyase is used to break down alginate or alginic acid and to reduce viscosity. It is useful during follicle isolation, encapsulation, and culture .

Biochem/physiol Actions

Alginate lyases actively participates in the saccharification of the acidic polysaccharides for the production of bioethanol.
Breaks down alginate or alginic acid to smaller molecules and reduces viscosity.

Unit Definition

One unit will produce an increase the A235nm of 1.0 per minute per mL of sodium alginate solution at pH 6.3 at 37 °C

Preparation Note

Add 0.15 mI of enzyme solution (1 un/ml) to 4.5 ml of 0.1% sodium alginate (pH 6.3). Incubate at 37 °C for 30 minutes. Terminate reaction by addition of 4.65 ml of 0.1 N NaOH.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Elizabeth M Parrish et al.
Reproduction (Cambridge, England), 142(2), 309-318 (2011-05-26)
Ovarian follicle maturation results from a complex interplay of endocrine, paracrine, and direct cell-cell interactions. This study compared the dynamic expression of key developmental genes during folliculogenesis in vivo and during in vitro culture in a 3D alginate hydrogel system.
Lena C E Lundqvist et al.
Carbohydrate research, 352, 44-50 (2012-03-21)
The gene coding for an alginate lyase from the marine bacteria Pseudomonas alginovora X017 was cloned and heterologously expressed in Escherichia coli strains. The protein was produced in inclusion bodies and the active form was obtained by applying a refolding
Adam J Wargacki et al.
Science (New York, N.Y.), 335(6066), 308-313 (2012-01-24)
Prospecting macroalgae (seaweeds) as feedstocks for bioconversion into biofuels and commodity chemical compounds is limited primarily by the availability of tractable microorganisms that can metabolize alginate polysaccharides. Here, we present the discovery of a 36-kilo-base pair DNA fragment from Vibrio
Yuya Morimoto et al.
APL bioengineering, 4(2), 026101-026101 (2020-04-09)
Biohybrid robots composed of biological and synthetic components have been introduced to reconstruct biological functions in mechanical systems and obtain better understanding of biological designs. For example, biohybrid robots powered by skeletal muscle tissue have already succeeded in performing various
Alginate lyase: Review of major sources and classification, properties, structure-function analysis and applications
Zhu B and Yin H
Bioengineered, 6(3), 125-131 (2015)

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