45253

Wright Stain solution

for microscopy

• Synonym(s): Eosin Methylene blue solution according to Wright, Wright’s eosin methylene blue
• MDL number: MFCD00005038
• UNSPSC Code: 12171500
• PubChem Substance ID: 329756625
• NACRES: NA.47

Properties

• grade: for microscopy
• shelf life: limited shelf life, expiry date on the label
• technique(s): microbe id | staining: suitable
• impurities: methanol
• density: 0.80 g/mL at 20 °C
• suitability: Chlamydia spp.; Histoplasma spp.; Plasmodium spp.; Trichomonas spp.; bacteria; protozoa; spirochetes
• SMILES string: [K+].COC(=O)c1ccccc1C2=C3C=C(Br)C(=O)C(Br)=C3Oc4c(Br)c([O-])c(Br)cc24
• InChI: 1S/C21H10Br4O5.K/c1-29-21(28)9-5-3-2-4-8(9)14-10-6-12(22)17(26)15(24)19(10)30-20-11(14)7-13(23)18(27)16(20)25;/h2-7,26H,1H3;/q;+1/p-1
• InChI key: NXBZXYJXNIFCRJ-UHFFFAOYSA-M

Description

General description

Wright Stain is a modified Romanowsky stain commonly used for the cellular elements of blood. Wright′s stain is a mixture of eosin Y with polychromed (oxidized and partly demethylated) methylene blue and therefore termed polychrome stains. The hematological stain is intended for differentially staining peripheral blood and bone marrow films.

Application

Wright stain has been used to determine the number of white blood cell types (neutrophils, lymphocytes, monocytes, eosinophils, and basophils) per microliter of whole blood.

Other Notes

Blood stain

Safety Information

• Pictograms: GHS02,GHS06,GHS08
• Signal Word: Danger
• Hazard Statements: H225 - H301 + H311 + H331 - H317 - H319 - H370
• Precautionary Statements: P210 - P280 - P301 + P310 + P330 - P302 + P352 + P312 - P304 + P340 + P311 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Skin Sens. 1 - STOT SE 1
• Target Organs: Eyes
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 3
• Flash Point(F): 51.8 °F
• Flash Point(C): 11 °C
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves