Chemphyschem : a European journal of chemical physics and physical chemistry, 6(5), 791-804 (2005-05-11)
Under high-excitation irradiance conditions in one- and two-photon induced fluorescence microscopy, the photostability of fluorescent dyes is of crucial importance for the detection sensitivity of single molecules and for the contrast in fluorescence imaging. Herein, we report on the dependence
The GTPase Ran is a key regulator of molecular transport through nuclear pore complex (NPC) channels. To analyze the role of Ran in its nuclear transport function, we used several quantitative fluorescence techniques to follow the distribution and dynamics of
Journal of the American Chemical Society, 136(31), 10965-10973 (2014-07-22)
This study highlights a crucial but often overlooked consideration during sample preparation involving surface-adsorbing species: the competition between analyte adsorption and analyte diffusion/mixing strongly affects the distribution of analytes throughout the sample. In cases of fast analyte adsorption, we argue
The detection of mercury in biological systems and its imaging is of highly importance. In this work, a ratiometric fluorescence sensor is developed based on fluorescence resonance energy transfer (FRET) with N-acetyl-L-cysteine functionalized quantum dots (NAC-QDs) as donor and Rhodamine
The aptamer (S2.2)-guided Ag-Au nanostructures (aptamer-Ag-Au) have been synthesized by photoreduction and validated by ultraviolet-visible light (UV-vis) spectra and transmission electron microscopy (TEM) images. Differential interference contrast (DIC), fluorescence, and TEM images, and surface-enhanced Raman scattering (SERS) spectra indicated that
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