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MABN707

Sigma-Aldrich

Anti-DCX Antibody, clone 2G5

clone 2G5, from mouse

Synonym(s):

Neuronal migration protein doublecortin, Doublin, Lissencephalin-X, Lis-X, DCX

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

2G5, monoclonal

species reactivity

mouse, human

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Dcx(13193)

Related Categories

General description

Doublecortin (DCX) or alternatively known as Doublin or Lissencephalin-X (Lis-X) and encoded by the human gene named DCX/DBCN/LISX is a microtubule associated protein that is required for the initial steps of neuronal dispersion and lamination during cortex brain development. Expressed by neuronal precursor cells and immature neurons, DCX expression is decreased by the time neurons are mature and express the mature neuronal marker NeuN. Thus DCX expression is a hallmark of developing neurons and neurogenesis. DCX expression is localized in the cytoplasm and along microtubule tracks. DCX appears to stabilize microtubules and causes bundling of them in the cytoplasm. In disease, DCX is the cause for an X-linked lissencephaly and subcortical laminar heterotopia, a disorder that is characterized by a “smooth” brain with few folds like normal brains and poorly formed cortical layers as well as other abnormalities. Besides developmental effects DCX may potentially help in the suppression of gliomas because in brain tumor stem cells (BTSC) self-renewal ability was significantly reduced in cells expressing DCX compared to control cells. EMD-Millipore’s Anti-DCX mouse monoclonal has been tested in western blot against recombinant protein as well as mouse heart lysate. The antibody has also been successfully tested in paraffin embedded immunohistochemistry on normal human brain and kidney cancer tissue sections as well as in fluorescent immunocytochemistry on HepG2 cells in culture and flow cytometry on SK-N-SH cells in culture and direct ELISA on varying concentrations of antigen.

Immunogen

Purified recombinant fragment of human DCX expressed in E. Coli.

Application

Detect Doublecortin using this mouse monoclonal antibody, Anti-DCX Antibody, clone 2G5 validated for use in western blotting, IHC, ICC & Flow Cytometry.
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected DCX in human brain and kidney cancer tissues.

Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected DCX in HepG2 cells.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected DCX in non serum starved SK-N-SH cells.

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience

Quality

Evaluated by Western Blotting in Mouse heart lysate.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected DCX in mouse heart lysate.

Target description

~50 kDa observed. Uncharacterized bands may appear in some lysate(s).

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal in PBS with up to 0.1% sodium azide and 0.5% protein stabilizer.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse heart lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Giulia Piccirilli et al.
Cellular and molecular neurobiology (2022-08-08)
Human cytomegalovirus (HCMV) causes congenital neurological lifelong disabilities. To date, the neuropathogenesis of brain injury related to congenital HCMV (cCMV) infection is poorly understood. This study evaluates the characteristics and pathogenetic mechanisms of encephalic damage in cCMV infection. Ten HCMV-infected human
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The Journal of pathology, 258(1), 69-82 (2022-06-11)
The development of neural structures within tumors is now considered vital for carcinogenesis. However, the time course of this development in human pre-invasive neoplasia has been incompletely described. Therefore, we performed a detailed analysis of nerves across the neoplastic spectrum
Jingjing He et al.
Scientific reports, 6, 34339-34339 (2016-10-13)
Parkinson's disease (PD) is one common neurodegenerative disease caused by a significant loss of midbrain dopaminergic neurons. Previous reports showed that 7, 8- dihydroxyflavone (7, 8-DHF) as a potent TrkB agonist can mimic BDNF and play neuroprotective roles for mouse
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Stem cell research & therapy, 12(1), 174-174 (2021-03-14)
Spinal cord injury (SCI) is a debilitating medical condition that can result in the irreversible loss of sensorimotor function. Current therapies fail to provide an effective recovery being crucial to develop more effective approaches. Mesenchymal stem cell (MSC) exosomes have
Christopher T Rhodes et al.
Cell cycle (Georgetown, Tex.), 17(3), 377-389 (2018-02-13)
Histone methyltransferases (HMTs) are present in heterogeneous cell populations within the adult brain including neurogenic niches. Yet the question remains whether loss of HMTs and the resulting changes in histone methylation alter cell fate in a region-specific manner. We utilized

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