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Key Documents

AB5453

Sigma-Aldrich

Anti-GABA A Receptor α6 Antibody

Chemicon®, from rabbit

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

Specificity

GABA A receptor alpha6 subunit. The apparent molecular mass of the alpha6 subunit in rat brain membrane fractions is 56-57 kDa. The antibody shows no reactivity in the forebrain of alpha6 knockout mice.

Immunogen

MBP fusion protein from the cytosolic loop of rat GABA A receptor alpha6 subunit.

Application

Anti-GABA A Receptor α6 Antibody detects level of GABA A Receptor α6 & has been published & validated for use in IH & WB.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Western blot: 1:1,000

Immunopreciptation: The antibody will immunoprecipitate 53% of all GABA A receptors in sodium dodecylsulfate extracts of rat forebrains (150 μL) using 15 μg of antibody.

Optimal working dilutions must be determined by the end user.

Physical form

Affinity purified immunoglobulin in 10 mM HEPES, pH 7.5, with 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Storage and Stability

Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self-defrosting freezer.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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James S Martenson et al.
eLife, 6 (2017-08-18)
GABAA receptor (GABAAR) pentamers are assembled from a pool of 19 subunits, and variety in subunit combinations diversifies GABAAR functions to tune brain activity. Pentamers with distinct subunit compositions localize differentially at synaptic and non-synaptic sites to mediate phasic and
Gili Ezra-Nevo et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 38(30), 6751-6765 (2018-06-24)
Corticotropin-releasing factor (CRF) and its type 1 receptor (CRFR1) play an important role in the responses to stressful challenges. Despite the well established expression of CRFR1 in granular cells (GrCs), its role in procedural motor performance and memory formation remains
Giovanna Ponti et al.
PloS one, 3(6), e2366-e2366 (2008-06-05)
Adult neurogenesis in mammals is restricted to some brain regions, in contrast with other vertebrates in which the genesis of new neurons is more widespread in different areas of the nervous system. In the mammalian cerebellum, neurogenesis is thought to

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