208902

Calpastatin Peptide

A 27-amino acid, cell-permeable peptide encoded by exon 1B of human calpastatin that acts as a potent inhibitor of calpain I and calpain II (IC₅₀ = 20 nM for purified rabbit calpain II).

• Synonym(s): Calpastatin Peptide, CS Peptide
• Empirical Formula (Hill Notation): C₁₄₂H₂₃₀N₃₆O₄₄S
• Molecular Weight: 3177.63
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• Quality Level: 100
• Assay: ≥95% (HPLC)
• form: lyophilized solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic)
• color: white
• solubility: 1% acetic acid: 1 mg/mL; water: 1 mg/mL
• shipped in: ambient
• storage temp.: −20°C

Description

General description

A 27-amino acid, cell-permeable peptide encoded by exon 1B of human calpastatin that acts as a potent inhibitor of calpain I and calpain II (IC₅₀ = 20 nM for purified rabbit calpain II). Does not inhibit either papain or trypsin. Blocks the down-regulation of protein kinase C_ε (PKC_ε) in rat pituitary GH₄C₁ cells stimulated by thyrotropin-releasing hormone (TRH).

A 27-amino acid, peptide encoded by exon 1B of human calpastatin that acts as a cell-permeable and potent inhibitor of calpain I and calpain II (IC₅₀ = 20 nM for purified rabbit calpain II). Does not inhibit either papain or trypsin. Blocks the down-regulation of protein kinase C_ε (PKC_ε) in rat pituitary GH4C1 cells stimulated by thyrotropin-releasing hormone (TRH).

Biochem/physiol Actions

Cell permeable: yes

Primary Target
calpain 2

Product does not compete with ATP.

Reversible: no

Target IC₅₀: 20 nM against calpain II

Warning

Toxicity: Standard Handling (A)

Sequence

Ac-Asp-Pro-Met-Ser-Ser-Thr-Tyr-Ile-Glu-Glu-Leu-Gly-Lys-Arg-Glu-Val-Thr-Ile-Pro-Pro-Lys-Tyr-Arg-Glu-Leu-Leu-Ala-NH₂

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Eto, A., et al. 1995. J. Biol. Chem. 270, 25115.
Kawasaki, J., et al. 1989. J. Biochem. 106, 274.
Maki, M., et al. 1989. J. Biol. Chem. 264, 18866.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable