810156P
Avanti
18:1-12:0 NBD PE
Avanti Research™ - A Croda Brand 810156P, powder
Synonym(s):
1-oleoyl-2-{12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]dodecanoyl}-sn-glycero-3-phosphoethanolamine
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About This Item
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Assay
>99% (TLC)
form
powder
packaging
pkg of 1 × 1 mg (810156P-1mg)
manufacturer/tradename
Avanti Research™ - A Croda Brand 810156P
shipped in
dry ice
storage temp.
−20°C
General description
N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) phosphoethanolamine (NBD PE) is a fluorescently labeled lipid where NBD is attached to the headgroup of phosphatidylethanolamine (PE). PE is an aminophospholipid abundant in brain.
Application
18:1-06:0 NBD PE or 1-oleoyl-2-{12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]dodecanoyl}-sn-glycero-3-phosphoethanolamine may be used as a component of liposome in cross-linking experiment and to determine M13 protein coat towards phospholipids.
Biochem/physiol Actions
N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) phosphoethanolamine (NBD PE) plays a key role as a fluorescent analog in the studies of organization and dynamics of membranes. NBD PE contributes to monitoring Red edge excitation shift (REES) effects, with the unique motional and dielectric properties it possesses.
Packaging
5 mL Amber Glass Screw Cap Vial (810156P-1mg)
Legal Information
Avanti Research is a trademark of Avanti Polar Lipids, LLC
Storage Class Code
11 - Combustible Solids
Certificates of Analysis (COA)
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Fluorescent methods to study biological membranes, 37-50 (2012)
Quantification of Protein-Lipid Selectivity using FRET: Application to the M13 Major Coat Protein
Journal of biophysics (Hindawi Publishing Corporation : Online), 87(1), 344-352 (2004)
Cardiolipin and phosphatidylethanolamine role in dibucaine interaction with the mitochondrial membrane
Biochimica et Biophysica Acta - Biomembranes, 1861(6), 1152-1161 (2019)
Biophysical journal, 87(1), 344-352 (2004-07-09)
Quantification of lipid selectivity by membrane proteins has been previously addressed mainly from electron spin resonance studies. We present here a new methodology for quantification of protein-lipid selectivity based on fluorescence resonance energy transfer. A mutant of M13 major coat
Organization and dynamics of N-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl)-labeled lipids: a fluorescence approach
Chemistry and Physics of Lipids, 127(1), 91-101 (2004)
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