White′s Basal Salt Mixture is widely used in plant tissue culture.[1]
Application
White′s Basal Salt Mixture has been used:
as a medium for the precultured spores of Prothalli of Adiantum capillus for the generation of protonemata[2]
to promote germination and cotton cotyledon unfolding[3]
as a solidified medium component for culturing A. capillus and as a component of selection medium for gametophyte selection after particle bombardment[4]
Formula variant
With the macro- and micronutrients as described by White (1968).
Methods in cell science : an official journal of the Society for In Vitro Biology, 22(4), 299-304 (2001-09-11)
An efficient novel method of direct somatic embryogenesis from basal tissue of garlic clove was developed. The influence of plant growth regulators, basal medium and explant type on somatic embryo induction was examined. The best plant growth regulator combination was
Methods in molecular biology (Clifton, N.J.), 1902, 35-45 (2018-12-14)
Biolistic transformation of cotton (Gossypium hirsutum L.) meristems, isolated from mature seed, is detailed in this report. A commercially available, helium-driven biolistic device (Bio-Rad PDS1000/He ) was used to bombard gold particles coated with a marker gene (uidA or "GUS")
RNA interference (RNAi) has become a powerful tool for determining gene function and is used in a wide variety of organisms. Since it is necessary to generate double-stranded RNA (dsRNA) as an inducer for RNAi, preparation of RNAi-inducing constructs is
Journal of experimental botany, 55(406), 2169-2177 (2004-09-14)
If, following an inductive treatment of 2 d of continuous darkness, shoot apices of Pharbitis nil are cultured 1 d later on White's medium supplemented with 2% sucrose, they cannot form carpels, but they can if they are cultured on
Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today.
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