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O7139

Sigma-Aldrich

Monoclonal Anti-Oligodendrocyte Marker O4 antibody produced in mouse

clone O4, purified immunoglobulin, lyophilized powder

Synonym(s):

Oligodendrocyte Marker O4 Antibody

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

O4, monoclonal

form

lyophilized powder

species reactivity

mouse, rat, chicken, human

technique(s)

flow cytometry: 0.25 μg/106 cells (rate differentiated cortical stem cells)
immunocytochemistry: 1-10 μg/mL

isotype

IgM

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... OMG(4974)

General description

Monoclonal anti-oligodendrocyte marker O4 was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with white matter of the corpus callosum from bovine brain. Oligodendrocytes are myelinating cells in the central nervous system (CNS) that form the myelin sheath of axons to support rapid nerve conduction.

Immunogen

white matter of corpus callosum from bovine brain.

Application

Monoclonal anti-oligodendrocyte marker O4 antibody produced in mouse has been used in immunocytochemistry and immunofluorescence.

Biochem/physiol Actions

Oligodendrocyte marker O4 functions as a development marker of oligodendrocytes. The monoclonal anti-oligodendrocyte marker O4 antibody has been used to describe a biologically distinct stage of the oligodendroglial lineage in-vitro.†

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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M2 microglia promotes neurogenesis and oligodendrogenesis from neural stem/progenitor cells via the PPAR? signaling pathway
Jichao Yuan
Oncotarget (2017)
Jichao Yuan et al.
Oncotarget, 8(12), 19855-19865 (2017-04-21)
Neural stem/progenitor cells (NSPCs) are an important source of cells for cell replacement therapy after nerve injury. How to induce NSPCs differentiation towards neurons and oligodendrocytes is a challenging issue in neuroscience research. In the present study, we polarized microglia
SDF-1a Secreted by Human CD133-Derived Multipotent Stromal Cells Promotes Neural Progenitor Cell Survival Through CXCR7
Benjamin Bakondi
Stem Cells and Development (2011)
Shuhui Yang et al.
ACS omega, 4(19), 18136-18146 (2019-11-14)
Neural stem cells (NSCs) play an important role in neural tissue engineering because of their capacity of self-renewal and differentiation to multiple cell lineages. The in vitro conventional neurosphere culture protocol has some limitations such as limited nutrition and oxygen
An efficient and economical culture approach for the enrichment of purified oligodendrocyte progenitor cells.
Niu J, et al.
Journal of Neuroscience Methods, 209, 241-249 (2012)

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