H6536
Monoclonal Anti-HIF-1α antibody produced in mouse
~1 mg/mL, clone H1α67, purified immunoglobulin, buffered aqueous solution
Synonym(s):
Anti-Hypoxia Inducible Factor 1, α subunit
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
conjugate:
unconjugated
application:
IHC (p)
IP
WB
IP
WB
clone:
H1α67, monoclonal
species reactivity:
rat, sheep, ferret, human, mouse
citations:
22
technique(s):
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500-1:1,000 using antigen retrieval
immunoprecipitation (IP): suitable
western blot: 1:500-1:1,000
immunoprecipitation (IP): suitable
western blot: 1:500-1:1,000
Pricing and availability is not currently available.
Recommended Products
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
H1α67, monoclonal
form
buffered aqueous solution
mol wt
antigen 120 kDa
species reactivity
rat, sheep, ferret, human, mouse
concentration
~1 mg/mL
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500-1:1,000 using antigen retrieval
immunoprecipitation (IP): suitable
western blot: 1:500-1:1,000
isotype
IgG2b
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... HIF1A(3091)
mouse ... Hif1a(15251)
rat ... Hif1a(29560)
General description
Hypoxia-inducible factor-1 (HIF-1) is a 120kD basic helix-loop-helix protein that belongs to the PAS superfamily of transcription factors. It has a crucial role in activating transcription of hypoxia-inducible genes. It can also activate the expression of the gene coding for NIP3; which subsequently induces cell apoptosis under persistent oxygen deprivation. Monoclonal anti-HIF-1α antibody can be used in immunostaining to detect HIF-1α localization. It can also be used in western blotting. Mouse anti-HIF-1α antibody reacts specifically with HIF1 α subunit of sheep, mouse, rat, ferret and human.
Immunogen
peptide corresponding to amino acids 432-528 of the human HIF-1α protein.
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Immunohistochemistry (1 paper)
Monoclonal anti-HIF-1α antibody (diluted 1: 500) can be used in immunofluorescence confocal microscopy. It can also be used in in immunoblotting, immunoprecipitation and immunohistochemistry.
Physical form
Solution in 0.02 M phosphate buffer containing 0.25 M sodium chloride and 0.1% sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class Code
10 - Combustible liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Lei Xiong et al.
The FEBS journal, 279(23), 4318-4326 (2012-10-09)
Several studies have identified a set of hypoxia-regulated microRNAs, among which is miR-210, whose expression is highly induced by hypoxia in various cancer cell lines. Recent studies have highlighted the importance of miR-210 and its transcriptional regulation by the transcription
Snider Desir et al.
Oncotarget, 7(28), 43150-43161 (2016-05-26)
In this study, we demonstrated that hypoxic conditions stimulated an increase in tunneling nanotube (TNT) formation in chemoresistant ovarian cancer cells (SKOV3, C200).We found that suppressing the mTOR pathway using either everolimus or metformin led to suppression of TNT formation
Yu Zhao et al.
Experimental and therapeutic medicine, 15(4), 4056-4060 (2018-03-28)
Hypoxia has been demonstrated to be a proangiogenic factor that induces vascular endothelial growth factor (VEGF) in retinal pigment epithelial (RPE) cells. Dickkopf2 (DKK2), originally known as Wnt antagonist, has recently been demonstrated to have an important regulatory role in
G L Semenza et al.
Kidney international, 51(2), 553-555 (1997-02-01)
Hypoxia-inducible factor 1 (HIF-1) is a basic helix-loop-helix protein that activates transcription of hypoxia-inducible genes, including those encoding: erythropoietin, vascular endothelial growth factor, heme oxygenase-1, inducible nitric oxide synthase, and the glycolytic enzymes aldolase A, enolase 1, lactate dehydrogenase A
Suchitra S Acharya et al.
Blood, 117(8), 2484-2493 (2010-12-18)
Joint arthropathy secondary to recurrent hemarthroses remains a debilitating complication of hemophilia despite the use of prophylactic factor concentrates. Increased vascularity and neoangiogenesis have been implicated in the progression of musculoskeletal disorders and tumor growth. We hypothesized that de novo
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
