dot blot: suitable immunofluorescence: suitable immunohistochemistry: suitable indirect ELISA: 1-2 μg/mL using using IAA conjugated to BSA western blot: suitable
isotype
IgG2b
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Auxin is a plant hormone that regulates physiological growth and development. This hormone regulates cellular functions such as elongation, turgor, division, and differentiation. Indoleacetic acid (IAA) is recognized as the main auxin in several plants. IAA is synthesized from tryptophan (Trp) using Trp dependent pathways,. Mouse monoclonal anti-auxin antibody recognizes indoleacetic acid but not free unmethylated IAA.
Immunogen
indoleacetic acid (IAA) carboxyl linked to ovalbumin.
Application
Indole-3-acetic acid was identified in developing P.abies spruce plant parrafin embedded formalin-fixed embryos by immunohistochemistry with the monoclonal anti-indole-3-acetic acid antibody.
Mouse monoclonal anti-auxin antibody has been used for immunofluorescence studies. The antibody can also be used for ELISA, dot blot and western blot analyses.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Proceedings of the National Academy of Sciences of the United States of America, 107(19), 8569-8574 (2010-04-28)
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International journal of molecular sciences, 24(13) (2023-07-14)
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