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57620

Sigma-Aldrich

Inulinase from Aspergillus niger

lyophilized, powder, brown-gray, ~25 U/mg

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About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

Aspergillus niger

Quality Level

form

powder

quality

lyophilized

specific activity

~25 U/mg

color

brown-gray

storage temp.

2-8°C

General description

Inulinases are classified as exoinulinases and endoinulinases.

Application

Inulinase from Aspergillus niger has been used as a glycoside hydrolase (GH) to study its potential to disperse bacteria (P. aeruginosa and S. aureus) from microbial biofilms using a polymicrobial well-plate model.

Biochem/physiol Actions

Inulinase hydrolyses inulin to produce oligosaccharides and liberate fructose. It also splits the terminal fructose units into sucrose and raffinose.
Inulinase is used in high-fructose syrup, which has therapeutic applications. Inulinase is reported to have high activity toward sucrose.

Unit Definition

1 U corresponds to the amount of enzyme which releases 1 μmol of reducing sugar (measured as fructose) per minute at pH 4.1 and 37°C from inulin

Other Notes

Characterization

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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R. Azhari et al.
Biotechnology and Applied Biochemistry, 11, 105-105 (1989)
Pierre Delanaye et al.
Clinica chimica acta; international journal of clinical chemistry, 413(5-6), 556-560 (2011-12-14)
Among issues susceptible to hamper a reliable measurement of inulin clearance, those regarding the dosage of inulin are largely neglected. We have compared the analytical performances of 2 commonly used methods of inulin dosage (one "acid" and one "enzymatic" method)
Takahiro Rikita et al.
Analytical biochemistry, 426(1), 24-26 (2012-04-10)
Inulin is a polysaccharide composed mainly of D-fructose units and is the most reliable indicator of the glomerular filtration rate. We have proposed an inulin detection method that involves the hydrolysis of inulin to D-fructose using inulinase and the selective
Gonçalo L M Santa et al.
Applied biochemistry and biotechnology, 165(1), 1-12 (2011-03-30)
The present work aims to provide the basic characterization of sol-gel immobilized inulinase, a biocatalyst configuration yet unexploited, using as model system the hydrolysis of inulin to fructose. Porous xerogel particles with dimensions in slight excess of 10 μm were obtained
Shinya Kuzuwa et al.
Gene, 495(2), 154-162 (2011-12-27)
Though some genetic features of lactobacillar fructan hydrolases were elucidated, information about their enzymology or mutational analyses were scarce. Lactobacillus casei IAM1045 exhibits extracellular activity degrading inulin. After partial purification of the inulin-degrading protein from the spent culture medium, several

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