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Key Documents

43388

Sigma-Aldrich

Esterase Isoenzyme 1 porcine liver, recombinant

recombinant, expressed in E. coli, ≥30.0 U/g

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About This Item

CAS Number:
Enzyme Commission number:
EC Number:
UNSPSC Code:
12352204
NACRES:
NA.54

recombinant

expressed in E. coli

Quality Level

form

crystalline
crystals
powder or flakes

specific activity

≥30.0 U/g

storage temp.

−20°C

Application

Esterase Isoenzymes may be used to identify the monocytic element in normal and leukemic cells. They may be used for the biochemical characterization of different hematopoietic cell lineages and stages of differentiation when studying leukemias and lymphomas. Esterase Isoenzyme 1, product 43388, is recombinant and from porcine liver. It is expressed in E. coli.

Biochem/physiol Actions

An esterase is a hydrolase that splits esters into acids and alcohols.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Unit Definition

1 U corresponds to the amount of enzyme which converts 1 μmol 4-nitrophenyl-L-acetate per minute at pH 7.5 and 30°C.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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H G Drexler et al.
Cancer, 59(1), 77-82 (1987-01-01)
The expression of a particular alpha-naphthyl acetate esterase isoenzyme which is specific for monocytes was examined in a panel of cultured leukemia-lymphoma cell lines (n = 88), freshly obtained leukemia-lymphoma cells (n = 527), and in fresh (n = 10)
Esterase isoenzyme profiles of 255 leukemia-lymphoma cell lines from all hematopoietic cell lineages.
SM Gignac, ZB Hu, et al.
Leukemia Lymphoma, 22, 143-151 (1996)
XieMei Tang et al.
Critical reviews in eukaryotic gene expression, 22(3), 179-187 (2012-11-13)
Tuberculosis remains one of the most prevalent and deadly infectious diseases, largely due to the emergence of multidrug-resistant and extensive drug-resistant Mycobacterium tuberculosis, especially the coinfection with HIV. Mycobacterium Ag85 complex (Ag85A, B, and C), with a carboxylesterase consensus sequence
Zhe-Yi Hu et al.
Analytical and bioanalytical chemistry, 405(5), 1695-1704 (2012-12-15)
Dabigatran etexilate (DABE) is an oral prodrug that is rapidly converted by esterases to dabigatran (DAB), a direct inhibitor of thrombin. To elucidate the esterase-mediated metabolic pathway of DABE, a high-performance liquid chromatography/mass spectrometry based metabolite identification and semi-quantitative estimation
Takashi Nishiyama et al.
Acta crystallographica. Section D, Biological crystallography, 69(Pt 1), 44-51 (2013-01-01)
Bacterial biofilm formation is an extremely widespread phenomenon involving the secretion of a protective exopolysaccharide matrix which helps the bacteria to attach to surfaces and to overcome a variety of stresses in different environments. This matrix may also include proteins

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