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O7380

Sigma-Aldrich

Orcein

Powder or crystals

Synonym(s):

Natural Red 28

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About This Item

CAS Number:
1400-62-0
EC Number:
215-750-6
MDL number:
MFCD00062310
UNSPSC Code:
12171500
NACRES:
NA.47

product name

Orcein, synthetic

biological source

synthetic

Quality Level

100

form

powder or crystals

composition

Carbon: 41.72%

Nitrogen: 2.23%

color

dark brown

solubility

1 M NaOH: 1 mg/mL, clear

εmax

≥400 at 580 nm in 0.1 M NaOH

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

InChI

1S/C28H24N2O7/c1-11-5-15(31)7-17(33)25(11)29-27-13(3)23-21(9-19(27)35)37-22-10-20(36)28(14(4)24(22)23)30-26-12(2)6-16(32)8-18(26)34/h5-10,29-34H,1-4H3

InChI key

VPEASJIRGSVXBF-UHFFFAOYSA-N

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General description

Orcein or CI Natural Red 28 is an elastic tissue stain. The fluorescence properties of Orcein show maximum emission peak at 587 nm (low concentration) and 620 nm (high concentration) upon suitable excitation and acidic pH.

Application

Orcein is highly recommended as a vital stain for elastic fibers and lamellae. Orcein is used as a nuclear stain for mounting embryos and assessing the maturation of oocytes. Other routine applications of orcein involve the staining of plant chromosomes.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCK5288
BCCH6378
BCCG9149
BCCF0062
BCCB7447

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M L Molero et al.
Journal of microscopy, 144(Pt 1), 45-53 (1986-10-01)
The fluorescence properties of orcein are described in this paper. Under suitable exciting light, acidic solutions of orcein showed emission peaks at 587 nm (low concentration) and at 620 nm (high concentration). In fluorescence microscopy an intense orange-red emission was
Mariusz Gajda et al.
Histochemistry and cell biology, 147(6), 671-681 (2017-02-09)
Numerous cellular and extracellular components should be analyzed in sections of atherosclerotic plaques to assess atherosclerosis progression and vulnerability. Here, we combined orcein (O) staining for elastic fibers and martius scarlet blue (MSB) polychrome to visualize various morphological contents of
Popescu P and Hayes H
Techniques in Animal Cytogenetics (2000)
The effects of L-ergothioneine and L-ascorbic acid on the in vitro maturation (IVM) and embryonic development (IVC) of sheep oocytes.
OZTURKLER Y
Development, 5-5 (2010)
Efficient decellularization of whole porcine kidneys improves reseeded cell behavior.
Poornejad N
Biomedical Materials (Bristol, England), 11, 025003-025003 (2016)
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