487655

p-Nitrophenyl Phosphate, DiTris Salt

Excellent substrate for alkaline phosphatase-based ELISA assays.

• Synonym(s): p-Nitrophenyl Phosphate, DiTris Salt, pNPP, 2Tris
• Empirical Formula (Hill Notation): C₁₄H₂₈N₃O₁₂P
• CAS Number: 68189-42-4
• Molecular Weight: 461.36
• MDL number: MFCD00037009
• UNSPSC Code: 41116158
• NACRES: NA.77

Properties

• Quality Level: 100
• Assay: ≥98% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• impurities: ≤0.05% Free-p-Nitrophenol
• color: off-white to pale yellow
• solubility: water: soluble
• shipped in: ambient
• storage temp.: 2-8°C
• SMILES string: [P](=O)([O-])([O-])Oc1ccc(cc1)[N+](=O)[O-].[N+H3]C(CO)(CO)CO.[N+H3]C(CO)(CO)CO
• InChI: 1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2
• InChI key: XXAXKCWOTRABOW-UHFFFAOYSA-N

Description

General description

Excellent substrate for alkaline phosphatase-based ELISA assays. Produces a yellow soluble end product that can be read at 405 nm to 410 nm. Both kinetic and end point determinations can be performed.

Excellent substrate for alkaline phosphatase-based ELISA assays. Produces yellow soluble end product that can be read at 405 nm to 410 nm.

Biochem/physiol Actions

Cell permeable: no

Primary Target
Substrate for alkaline phosphatase-based ELISA assays

Product does not compete with ATP.

Reversible: no

Warning

Toxicity: Standard Handling (A)

Reconstitution

Unstable in solution; reconstitute just prior to use.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable