RAB0631

Human C5a / Complement C5a ELISA Kit

for serum, plasma, and cell culture supernatants

• Synonym(s): Complement C5a
• UNSPSC Code: 41116158
• NACRES: NA.32

Properties

• species reactivity: human
• packaging: kit of 96 wells (12 strips x 8 wells)
• technique(s): ELISA: suitable
• input: sample type plasma; sample type cell culture supernatant(s); sample type serum
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 31 pg/mL; standard curve range: 31.25-2000 pg/mL
• detection method: colorimetric
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... C5(727)

Description

General description

Human complement 5a (C5a) is generated during complement activation from the α-chain of complement C5. It binds to a signaling G-protein coupled receptor (GPCR), inducing neutrophil chemotaxis and endothelial cell activation. C5a also triggers an oxidative burst in macrophages and neutrophils and induces release of histamine in basophils and mast cells.

The antibody pair provided in this kit recognizes human Complement Component C5a.

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

• RABTMB3 ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
• RABSTOP3 ELISA Stop Solution (Item I)
• RABELADA ELISA 1X Assay/Sample Diluent Buffer A (Item D1)
• RABELADB ELISA 5X Assay/Sample Diluent Buffer B (Item E1)
• RABWASH4 20X Wash Buffer (Item B)

Safety Information

• Pictograms: GHS05
• Signal Word: Warning
• Hazard Statements: H290
• Precautionary Statements: P234 - P390
• Hazard Classifications: Met. Corr. 1
• Storage Class Code: 8A - Combustible corrosive hazardous materials
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable