P3415

3-(2-Pyridyldithio)propionic acid N-hydroxysuccinimide ester

≥95%, powder

• Synonym(s): N-Succinimidyl 3-(2-pyridyldithio)propionate, SPDP
• Empirical Formula (Hill Notation): C₁₂H₁₂N₂O₄S₂
• CAS Number: 68181-17-9
• Molecular Weight: 312.36
• Beilstein: 1548137
• EC Number: 269-034-3
• MDL number: MFCD00009636
• UNSPSC Code: 12352106
• PubChem Substance ID: 24898409
• NACRES: NC.07

Properties

• Assay: ≥95%
• form: powder
• reaction suitability: reagent type: cross-linking reagent
• mp: 84-86 °C (lit.)
• solubility: acetone: 50 mg/mL; DMF: soluble
• functional group: NHS ester
• storage temp.: −20°C
• SMILES string: O=C(CCSSC1=NC=CC=C1)ON2C(CCC2=O)=O
• InChI: 1S/C12H12N2O4S2/c15-10-4-5-11(16)14(10)18-12(17)6-8-19-20-9-3-1-2-7-13-9/h1-3,7H,4-6,8H2
• InChI key: JWDFQMWEFLOOED-UHFFFAOYSA-N

Description

General description

3-(2-Pyridyldithio)propionic acid N-hydroxysuccinimide ester (SPDP) is a heterobifunctional cross-linking reagent with amine and sulfhydryl reactivity. It is typically coupled initially to molecules containing primary amines by amide bonds buffered at pH 7.5 (6.5-8.5). Second coupling specific for molecules containing free sulfhydryl by thiol-disulfide exchange buffered at pH 8.0 (7.5-8.5). Alternately, this reagent may be used as a protected thiolating reagent following initial coupling and reduction of linker. It incorporates four atom linker.

Application

3-(2-Pyridyldithio)propionic acid N-hydroxysuccinimide ester (SPDP) is useful for the preparation of enzyme immunoconjugates and hapten carrier molecule conjugates.
It has been used for antibody conjugation and as a protein cross-linker.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves