M6908

Myeloperoxidase from human leukocytes

lyophilized powder, ≥50 units/mg protein

• Synonym(s): MPO, Peroxidase, myelo
• CAS Number: 9003-99-0
• Enzyme Commission number: 1.11.2.2 (BRENDA, IUBMB)
• MDL number: MFCD00131641
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human leucocytes
• Quality Level: 200
• form: lyophilized powder
• specific activity: ≥50 units/mg protein
• solubility: H₂O: soluble; aqueous buffers such as 50 mM sodium acetate: soluble (Freezing of the enzyme in solution may result in a substantial loss of activity.)
• UniProt accession no.: P05164
• shipped in: dry ice
• storage temp.: −20°C
• InChI: 1S/H2O3/c1-3-2/h1-2H
• InChI key: JSPLKZUTYZBBKA-UHFFFAOYSA-N
• Gene Information: human ... MPO(4353)

Description

General description

Myeloperoxidase (MPO) is encoded by the gene mapped to human chromosome 17q22-24. The encoded protein is a leukocyte-derived enzyme and is an integral constituent of the innate immune response. MPO belongs to the heme peroxidase superfamily. MPO is a heme protein containing iron and is highly expressed in leukocytes, neutrophils, monocytes and some subtypes of tissue macrophages.

Application

Myeloperoxidase (MPO) from human leukocytes has been used in MPO assay.

Myeloperoxidase from human leukocytes has been used:

• as a standard to quantify the neutrophil (polymorphonuclear cell [PMN]) infiltration in lung tissue
• as a neutrophil extracelullar trap (NET) component to stimulate 10⁶ dendritic cells or 10⁶ macrophages
• as a standard in myeloperoxidase (MPO) assay to measure pancreatic MPO

Biochem/physiol Actions

Myeloperoxidase (MPO) has a crucial role to play in destruction of various microorganisms and foreign cells, such as bacteria, fungi, viruses, red cells and malignant and nonmalignant nucleated cells. MPO catalyzes the production of number of reactive oxidant species (ROS) that influence tissue damage during inflammation. MPO and its downstream inflammatory pathways function as a potent therapeutic target for prophylaxis of atherosclerotic cardiovascular disease. MPO catalyze the synthesis of polychlorinated dibenzo(p)dioxins and furans (PCDD/F) from precursor such as chlorophenols in presence of hydrogen peroxide (H₂O₂).

Other Notes

Lysosomal heme-containing protein.

Unit Definition

One unit of myeloperoxidase will catalyze the consumption of one micromole of hydrogen peroxide and the production of ¹/₄ micromole of tetraguaiacol per minute at pH 7.0 and 25 °C

Physical form

Lyophilized from 50 mM sodium acetate buffer, pH 6.0, 0.1 M sodium chloride

Analysis Note

Source material tested and found negative for antibody to HIV and for HBSAG.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable