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GERPN2235

ECL™ Select Western Blotting Detection Reagent

Cytiva RPN2235

Synonym(s):

chemiluminescent detection reagent, Western blotting detection reagent

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About This Item

UNSPSC Code:
41105332
NACRES:
NA.32

packaging

pkg of 1 kit

manufacturer/tradename

Cytiva RPN2235

General description

Amersham™ ECL™ Select: Western blotting detection reagent with highly intense signal output, for detection of medium to very low protein levels.

Amersham™ ECL™ Select is a high sensitivity reagent for chemiluminescent Western blotting detection. High signal intensity makes Amersham™ ECL™ Select suitable for the most demanding Western blotting applications, allowing for detection of minute protein quantities. Its long lasting signals allow for multiple exposures and a convenient time window between experiment and analysis. Signals can be detected with CCD camera or X-ray film.

Amersham™ ECL™ Select typically exhibits a dynamic range that is complementary to Amersham™ ECL™ Prime, and similar to Amersham™ ECL™ Advance.

Application

Western Blotting

Features and Benefits

  • Improved sensitivity: Produces a very high signal intensity
  • Quantitative measurements: Linear dynamic range allowing for quantitation of medium to very low abundant proteins
  • Works with a wide range of antibody dilutions, reducing the need for lengthy optimization
  • Optimized for imaging with ImageQuant LAS 4000 (CCD-based imaging) and compatible with Amersham™ Hyperfilm
  • Compatible with Rainbow™ Molecular Weight Markers and Amersham™ ECL™ DualVue Western Blotting Markers.
  • Optimized for use with Amersham™ Hybond-P (PVDF) membranes and compatible with Amersham™ Hybond® ECL™ (nitrocellulose) membranes

Components

includes
Solution A (Luminol solution, 50 mL)
Solution B (Peroxide solution, 50 mL)
sufficient for 1000 cm2 membrane

complete packsize
for 1000 cm2 membrane

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Amersham is a trademark of Cytiva
ECL is a trademark of Cytiva
Hybond is a registered trademark of Cytiva
Rainbow is a trademark of Cytiva

Storage Class Code

12 - Non Combustible Liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Methods Used to Detect Proteins and Nucleic Acids Bound to Membranes

Background and protocols describing the various methods used by molecular biologists to detect samples of protein or nucleic acids bound to membranes.

Troubleshooting Detection Methods for GST-tagged Proteins

This page describes troubleshooting strategies in the detection of GST-tagged proteins.

Protocols

Chemiluminescence Detection with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select

This page describes chemiluminescence detection of GST-tagged proteins with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select from Cytiva.

Detection of GST-tagged Proteins with Western Blot

This page describes how to monitor expression and purification of GST-tagged proteins by Western blot analysis using Cytiva products.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service