This product is stable for approximately 5 years in powder form with the vial unopened. It is guaranteed for 1 year from the date of shipment. The stability upon reconstitution has not been determined. However, for a similar product, it is recommended to aliquot and freeze at -20°C after reconstitution. Stock solutions are stable for up to 2 months at -20°C and should be protected from light. Degradation occurs gradually and depends on exposure frequency and intensity. Discolored, cloudy, or particulate-containing solutions should be discarded. Solutions can be sterile filtered using a 0.22 µm membrane, as the powders are not sterile.
D4902
Dexamethasone
Dexamethasone
powder, BioReagent, suitable for cell culture, ≥97%
Synonym(s):
(11β,16α)-9-Fluoro-11,17,21-trihydroxy-16-methylpregna-1,4-diene-3,20-dione, 9α-Fluoro-16α-methyl-11β,17α,21-trihydroxy-1,4-pregnadiene-3,20-dione, 9α-Fluoro-16α-methylprednisolone, Prednisolone F
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All Photos(5)
About This Item
Empirical Formula (Hill Notation):
C22H29FO5
CAS Number:
Molecular Weight:
392.46
Beilstein:
2066651
EC Number:
MDL number:
UNSPSC Code:
12352209
PubChem Substance ID:
NACRES:
NA.75
biological source:
synthetic
form:
powder
Assay:
≥97%
Pricing and availability is not currently available.
Recommended Products
biological source
synthetic
Quality Level
product line
BioReagent
Assay
≥97%
form
powder
potency
4-500 ng/mL
technique(s)
cell culture | mammalian: suitable
single cell analysis: suitable
mp
262-264 °C (lit.)
solubility
methanol: 25 mg/mL
shipped in
ambient
storage temp.
2-8°C
SMILES string
C[C@@H]1C[C@H]2[C@@H]3CCC4=CC(=O)C=C[C@]4(C)[C@@]3(F)[C@@H](O)C[C@]2(C)[C@@]1(O)C(=O)CO
InChI
1S/C22H29FO5/c1-12-8-16-15-5-4-13-9-14(25)6-7-19(13,2)21(15,23)17(26)10-20(16,3)22(12,28)18(27)11-24/h6-7,9,12,15-17,24,26,28H,4-5,8,10-11H2,1-3H3/t12-,15+,16+,17+,19+,20+,21+,22+/m1/s1
InChI key
UREBDLICKHMUKA-CXSFZGCWSA-N
Gene Information
human ... ABCB1(5243) , CYP3A4(1576) , IL4(3565) , IL5(3567) , NR3C1(2908)
mouse ... Abcb1a(18671) , Abcb1b(18669) , Ifng(15978) , Nos2(18126) , Ptgs2(19225) , Tnf(21926)
rat ... Ar(24208) , Nr3c1(24413) , Tnf(24835)
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Application
Biochem/physiol Actions
Glucocorticoid anti-inflammatory agent
Glucocorticoid anti-inflammatory agent. Regulates T cell survival, growth, and differentiation. Inhibits the induction of nitric oxide synthase.
It is poorly metabolized in the body and has great affinity and bioavailability.[6]
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Physical form
powder 2-8 °C; stock-frozen in working aliquots, avoid repeated freeze/thaw
Reconstitution
To prepare 20 μg/ml stock solution, add 1ml absolute ethanol per mg product; gently swirl to dissolve. Add 49 ml sterile medium per ml of ethanol added, while mixing, to acheive final concentration of 20 μg/ml.
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Product No.
Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Repr. 1B
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Protocols
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Discover our primary human hepatocytes and plating and maintenance media formulations. Explore our protocol for plateable primary hepatocyte cell culture.
Discover our primary human hepatocytes and plating and maintenance media formulations. Explore our protocol for plateable primary hepatocyte cell culture.
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Two questions: 1. How to store 1mg/ml solution and how stable? 2. Is it advisable to filter working solution 20ug/ml with 0.22 um prior to use?
1 answer-
Helpful?
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Hello, I prepared a solution of Dexamethasone in DMSO and PBS for IP injection in mice for 20 days daily. Is it okay to store the solution at 4°C?
1 answer-
Dexamethasone solutions in DMSO are stable for at least 30 days when stored at 4 °C and protected from light. Do not dilute to working concentrations in an aqueous buffer or medium until ready to use.
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Can I store D4902 at -80°C? If I store it at -80°C instead of -20°C, can I use it stably for more than 2 months?
1 answer-
Long-term solution stability studies have not been performed on this material. The impact of stability at -80°C has not been determined. However, an analytical standard available at 1 mg/mL in methanol, as product D-085, has a shelf life of 4 years when stored at -20°C. In addition, therapeutic preparations of Dexamethasone 3.3 mg/mL for injection manufactured by Hospira, list a shelf life of 24 months at -20°C. This product, in solution, may demonstrate similar stability.
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What is the solubility of D4902 (Dexamethasone) in DMSO and ethanol? Additionally, how should stock solutions be stored?
1 answer-
The solubility of D4902 (Dexamethasone) in ethanol is 25 mg/ml and DMSO is 25 mg/ml. Dexamethasone is insoluble in water. Stock solutions are stable for up to 2 months at -20°C.
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IF Dexamethasone solutions forgrt to protected from light, what will happen in these solutions?
1 answer-
Prepared solutions of Dexamethasone should be protected from light. Degradation occurs gradually and is dependent on the frequency and intensity of exposure. Solutions that appear discolored, cloudy, or contain particulates should be discarded. Unfortunately, there is not way to determine the degree of decomposition.
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I read that the dexamethasone is packaged in a bottomless glass bottle, in an inserted fused cone. Is this cone sealed with a rubber lid to be able to safely open the bottle and dissolve all contents before fully opening the bottle?
1 answer-
Please see the attached images of the vial and cap used to package this product. The cap is a screw-type and does not include a septum. Threading secures the inner cone to the outer vial.
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what are different between D4902 and D1756? and if the same the both, why D4902 is descriptive about 'suitable for cell culture'?
1 answer-
Product D4902 and D1756 are identical compounds, but are tested differently. Product D4902 is tested specifically for suitability in cell culture applications, while the D1756 has a slightly higher minimum purity specification. Please see the sample Certificate of Analysis to view the testing and specifications for each product:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/D1756-BULKBCCK0031.pdf
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/D4902-BULKBCCJ0003.pdfHelpful?
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Are there any references for D2915?
1 answer-
Please check the following offerings for Certified Reference standards for Dexamethasone:
https://www.sigmaaldrich.com/product/sial/d0700000
https://www.sigmaaldrich.com/product/sial/phr1526
https://www.sigmaaldrich.com/product/usp/1176007Helpful?
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Can I use Product D4902 as a local application (after preparation) to induce osteogenesis in a human clinical trial?
1 answer-
This product is suitable for cell culture application and is intended for research use only. For more information on "Use of Products", please consult our Terms and Conditions https://www.sigmaaldrich.com/US/en/life-science/legal/terms-and-conditions#3
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Can I use Product D4902, Dexamethasone for humans as a local agent that is applied in the dental socket as an osteogenic inducer? I read an article about this product being used in the dental sockets of rabbits with positive results.
1 answer-
This product is not intended for human or animal use; it is sold for research use only. Please see our Terms and Conditions regarding use of products here: https://www.sigmaaldrich.com/life-science/legal/terms-and-conditions#3
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