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D3035

Sigma-Aldrich

Deoxyribonucleic acid from human placenta

Deoxyribonucleic acid from human placenta

buffered aqueous solution, sexed, female

Synonym(s):

DNA

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About This Item

CAS Number:
9007-49-2
MDL number:
MFCD00130921
UNSPSC Code:
41106310
NACRES:
NA.52
Pricing and availability is not currently available.

biological source

human placenta

Quality Level

200

grade

for molecular biology

form

buffered aqueous solution

shipped in

dry ice

storage temp.

−20°C

SMILES string

[P](=O)(OC2C(OC(C2)N)CO[P](=O)(OC3C(OC(C3)N)CO)O)(OCC1OC(CC1O)N)O

InChI

1S/C15H31N3O13P2/c16-13-1-7(20)11(28-13)5-25-32(21,22)31-9-3-15(18)29-12(9)6-26-33(23,24)30-8-2-14(17)27-10(8)4-19/h7-15,19-20H,1-6,16-18H2,(H,21,22)(H,23,24)

InChI key

AWBASQCACWFTGD-UHFFFAOYSA-N

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General description

Human placental DNA is isolated from a single female donor placenta, but will contain some maternal DNA. The sex has been determined using hybridization with DNA probes.

Application

Deoxyribonucleic acid from human placenta has been used:
  • to compare the efficiency of DNA quantification methods[1]
  • as a standard in real-time PCR analysis of DNA samples from bladder cancer cell lines[2]
  • as an internal control to estimate the degree of fragmentation of circulating DNA[3]
  • for restriction digest analysis to identify low copy repeat regions of human chromosome 22q11[4]
  • to compare the 70-bp P5 exon sequence between DNA of different human and primate species[5]
  • to calculate copy number of genes, relative to normal human tissue[6]
  • in PCR reactions[7]
For use in Southern hybridizations.

Recommended products

Solutions of DNA have been stored successfully for several months at 4 C, in 10 mM Tris, pH 7.5 - 8.0, with 1 mM EDTA and without a bacteriostatic agent. At low concentrations, about 25 μg/ml, DNA tends to absorb onto the surfaces of plastic tubes.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
123K37971
123K3797
089K3789
089K3788
089K3787

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Alain R Thierry et al.
Nucleic acids research, 38(18), 6159-6175 (2010-05-25)
Although circulating DNA (ctDNA) could be an attractive tool for early cancer detection, diagnosis, prognosis, monitoring or prediction of response to therapies, knowledge on its origin, form and rate of release is poor and often contradictory. Here, we describe an
Joanne S Aveyard et al.
The Journal of molecular diagnostics : JMD, 6(4), 356-365 (2004-10-28)
Many tumors have large homozygous deletions of the CDKN2A locus (encoding p14(ARF) and p16) and of CDKN2B (p15). Our aim was to determine which gene is the major target in bladder cancer. We used quantitative real-time PCR (RTQ-PCR) to determine
J E Collins et al.
Genome research, 7(5), 522-531 (1997-05-01)
A clone map consisting of YACs, cosmids, and fosmids has been constructed covering low copy repeat regions of human chromosome 22q11. A combination of clone restriction digest analysis, single-copy landmark content analysis, HindIII-Sau3AI fingerprinting, and sequencing of PCR products derived
Heather Hoover et al.
Journal of proteome research, 14(9), 3670-3679 (2015-07-08)
Tumor types can be defined cytologically by their regions of chromosomal amplification, which often results in the high expression of both mRNA and proteins of certain genes contained within the amplicon. An important strategy for defining therapeutically relevant targets in
Claire E L Smith et al.
PloS one, 12(9), e0185678-e0185678 (2017-09-29)
The imprinted gene PLAGL1 is an important regulator of apoptosis and cell cycle arrest. Loss of its expression has been implicated in tumorigenesis in a range of different cancers, and overexpression during fetal development causes transient neonatal diabetes mellitus (TNDM).
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Questions

  1. Hi, Could you please clarify what is the concentration (and the volume) of the D3035 product. Thank you

    1 answer

    1. The concentration will be listed on the lot specific Certificate of Analysis. Please navigate to the ‘DOCUMENTATION’ section of the Product Detail Page to access a Certificate:
      https://www.sigmaaldrich.com/product/sigma/d3035#product-documentation

      The volume will depend on the pack size ordered and the concentration.
      For instance, a 0.5 mg pack at 0.05 mg/ml will have a volume of at least 10ml.

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