CA200

cAMP Enzyme Immunoassay Kit, Direct

• Synonym(s): cAMP detection kit
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• technique(s): ELISA: suitable
• Quality Level: 200
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Adenosine 3′,5′-cyclic monophosphate (cAMP) is a second messenger in many physiological processes. It is synthesized from adenosine triphosphate (ATP) in the presence of enzyme adenylate cyclase.

Non-radioactive, competitive immunoassay for the quantitation of total cAMP in tissue and cell cultures treated with .1M HCL.The kit cAMP uses a cAMP polyclonal antibody to competitively bind the cAMP in the sample or alkaline phosphatase moleule that has cAMP covalently attachd to it.

Application

Please refer to the attached Protocolfor details.

cAMP Enzyme Immunoassay Kit, Direct has been used to measure cAMP levels in cell culture medium virulent and attenuated macrophages. It has also been used to quantify cAMP synthesis in olfactory epithelium.

Biochem/physiol Actions

Adenosine 3′,5′-cyclic monophosphate (cAMP) is shown to be involved in the cardiovascular and nervous systems, immune mechanisms, cell growth and differentiation, and general metabolism. Deciphering cAMP modulation in regulatory pathways may help in understanding of the physiology and pathology of many disease states.

Other Notes

Allow reagents to warm at room temperature for 30 minutes prior to opening.

Kit Components Only

• 0.1 M Hydrochloric Acid 1 x 30
• Acetic Anhydride 1 x 1
• cAMP-Alkaline Phosphatase Conjugate 1 x 5
• cAMP EIA Antibody Rabbit Anti-cAMP 1 x 5
• 5 Cycle Logit-Log Paper 1 x 1
• Cyclic AMP Standard 1 x 0.5
• Direct cAMP Assay Layout Sheet 1 x 1
Goat Anti-Rabbit IgG Coated 96 Well Microtiter Plate 1 ea
Neutralizing Reagent 1 x 6
p-Nitrophenyl Phosphate Substrate Solution 1 x 20
Plate Sealer 1 ea
Stop Solution 1 x 5
Triethylamine 1 x 2
Wash Buffer Concentrate 1 x 30

See All (14)

Safety Information

• Pictograms: GHS02,GHS06,GHS05
• Signal Word: Danger
• Hazard Statements: H226,H290,H302,H311,H314,H330,H335
• Precautionary Statements: P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1A - STOT SE 3
• Target Organs: Respiratory system
• Supplementary Hazards: EUH071
• Storage Class Code: 3 - Flammable liquids
• Flash Point(F): 120.2 °F
• Flash Point(C): 49 °C