C6870

Anti-COMT antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): Anti-Catechol-O-methyltransferase
• MDL number: MFCD03454916
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~24 kDa (and ~28 kDa)
• species reactivity: human, mouse, rat
• technique(s): immunoprecipitation (IP): 5-10 μL using human HepG2 cell lysates; western blot: 1:250-1:500 using whole extracts of mouse and rat liver
• UniProt accession no.: P21964
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... COMT(1312) ; mouse ... Comt1(12846) ; rat ... Comt(24267)

Description

General description

Catechol-O-methyltransferase (COMT) is encoded by the gene mapped to human chromosome 22q11.21. It is widely expressed in neuronal and non-neuronal tissues. In mammals, two isoforms of COMT have been identified a cytosolic soluble form (S-COMT) and a membrane-bound form (MB-COMT), whose relative levels differ in various tissues and species.

Specificity

The antibody recognizes MB-COMT and S-COMT.

Application

Anti-COMT antibody produced in rabbit has been used in enzyme linked immunoassay (ELISA).

Anti-COMT antibody produced in rabbit is suitable for immunoprecipitation at a concentration of 5-10μL using human HepG2 cell lysates and western blotting at a dilution of 1:250-1:500 using whole extracts of mouse and rat liver.

Biochem/physiol Actions

Catechol-O-methyltransferase (COMT) catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamine neurotransmitters including dopamine, noradrenaline, and adrenaline, their metabolites, and L-DOPA. Its major physiological role is to inactivate biologically active or toxic catechols. Variations in enzymatic activity of COMT result in the pathogenesis of different psychiatric and neurological diseases. COMT inhibitors are important therapeutic agents used in the treatment of Parkinson′s disease.

Catechol-O-methyltransferase (COMT) is referred as HEL-S-98n. The COMT Met/Met genotype individuals can acquire skill in executive function tasks (as multi-UV supervisory control to a higher level and faster than other genotype groups). The genetic variants of COMT alter neurobehavioral functions that are affected by mercury in adults and might modify the adverse neurobehavioral effects of mercury exposure in children.

Target description

COMT (Catechol-O-methyltransferase) catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves