Coconut water is used as a supplement with plant cell culture media such as Murashige & Skoog media.
Use at a concentration of 5-20% (v/v)
Other Notes
Coconut water has been shown to stimulate shoot proliferation in many species of plants. It is prepared from selected coconuts and processed to remove most of the protein. The product is then filter sterilized and frozen prior to shipment. Remaining protein levels in the water may vary from one lot to the next and may result in precipitate when the product is frozen. This precipitation should not effect the growth of the plant tissue. The precipitate can be removed by filtering or by allowing it to settle to the bottom of the bottle and then decanting. Coconut water can be divided into smaller aliquots, corresponding to your standard medium batch size, and refrozen until needed. Coconut water should be used at a concentration of 5-20% (v/v).
Indian journal of experimental biology, 43(3), 280-285 (2005-04-09)
Large-scale in vitro propagation protocol for Dendrobium hybrids Sonia 17 and 28, two highly prized commercial cut flower cultivars through shoot multiplication using flower stalk node explants and protocorm-like bodies (PLBs) formation was accomplished. Both hybrids did not exhibit significant
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Indian journal of experimental biology, 46(4), 243-248 (2008-06-03)
In vitro mass production of C. suaveolens (Lindl.) Hook, an endangered orchid with its snowy white flowers having horticultural potential was accomplished through immature seed culture, and subsequent plant regeneration. The developmental stage of the immature seeds and nutrient media
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Preparation from Packaged Powder, Preparation from Basal Salt Solutions, Banana Powder Preparation and Use, Coconut Water Preparation and Use
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