In order to achieve 2% BSA, dissolve 100 mL of PBS in 2 G of BSA. So, dissolving 20 G of BSA in 1000 mL of PBS would give 2% BSA.
A5611
Bovine Serum Albumin
Cohn Fraction V, lyophilized powder, ≥96% (agarose gel electrophoresis)
Synonym(s):
Albumin bovine serum, BSA, Bovine albumin
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About This Item
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biological source
bovine
Quality Level
Assay
≥96% (agarose gel electrophoresis)
form
lyophilized powder
mol wt
~66 kDa
packaging
poly bottle of
origin
USA origin
technique(s)
immunohistochemistry: suitable
pH
4.8-7.5
solubility
H2O: soluble 40 mg/mL, clear to slightly hazy, yellow
UniProt accession no.
storage temp.
2-8°C
Gene Information
bovine ... ALB(280717)
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General description
Application
Biochem/physiol Actions
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
Preparation Note
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
This is a cold alcohol fractionated albumin that is produced by Sigma-Aldrich with a stricter adherence to the original Cohn method of fractionation than other commercially available bovine albumins.
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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How can I prepare 1L of PBS with 2% of BSA
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