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32670
4′,6-Diamidino-2-phenylindole dihydrochloride
4′,6-Diamidino-2-phenylindole dihydrochloride
suitable for fluorescence, BioReagent, ≥95.0% (HPLC)
Synonym(s):
2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride, DAPI dihydrochloride
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About This Item
Empirical Formula (Hill Notation):
C16H15N5 · 2HCl
CAS Number:
Molecular Weight:
350.25
Beilstein:
4894417
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.32
Pricing and availability is not currently available.
Recommended Products
product line
BioReagent
Quality Level
Assay
≥95.0% (HPLC)
form
powder
solubility
DMF: soluble
H2O: soluble
fluorescence
λex 340 nm; λem 488 nm (nur DAPI)
λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)
λex 374 nm; λem 461 nm in 10 mM Tris; 1 mM EDTA; pH 8.0; DNA
suitability
suitable for fluorescence
storage temp.
room temp
SMILES string
Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N
InChI
1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
InChI key
FPNZBYLXNYPRLR-UHFFFAOYSA-N
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Application
DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels.
DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.
Biochem/physiol Actions
Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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What is the Department of Transportation shipping information for this product?
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What concentration of 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), Product 32670, is used to as a nuclear counterstain for tissue sections?
1 answer-
Following fluorescent staining of the cells, DAPI is added to the mounting media at 2 μg/ml.
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What concentration of 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), Product 32670, is used to as a nuclear stain for cultured cells?
1 answer-
Use DAPI at 0.1 microgram per mL in phosphate buffered saline (PBS) to incubate with monolayer cultured cells for 15-30 minutes at 30°C. Immediately examine with a fluorescence microscope.
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How can I store solutions of 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), Product 32670?
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At 1 mg/mL, the solutions are stable in the dark at 2-8 °C for several weeks. For long-term storage, the solutions can be divided into aliquots and stored frozen at -20°C
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How can I make a solution of 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), Product 32670?
1 answer-
DAPI is soluble in water (10 mg/mL) with heat or sonication as necessary. It is insoluble in PBS even at 1 mg/ml.
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