11685619910
Roche
Fluorescein RNA Labeling Mix
solution, suitable for Northern blotting, suitable for Southern blotting, suitable for hybridization
Synonym(s):
RNA labeling
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
41105500
Recommended Products
form
solution
Quality Level
usage
sufficient for 20 reactions
packaging
pkg of 40 μL
manufacturer/tradename
Roche
technique(s)
Northern blotting: suitable
Southern blotting: suitable
hybridization: suitable
storage temp.
−20°C
General description
Fluorescein RNA Labeling Mix is a convenient nucleotide mixture for the labeling of RNA with fluorescein-12-uridine triphosphate (UTP).
Specificity
Heat inactivation: Stop the reaction by adding 2μl 0.2M EDTA (pH 8.0).
Application
Fluorescein-labeled RNA has been used in a variety of hybridization techniques such as:
- in situ hybridization to tissues
- Southern blots
- northern blots
- plaque or colony lifts
- RNase protection experiments
Quality
The mix is function tested in combination with the DIG RNA Labeling Kit.
Principle
Fluorescein-labeled, single-stranded RNA probes of defined length are generated by in vitro transcription. Fluorescein-12-UTP is incorporated by SP6, T7, and T3 RNA polymerases at approximately every 20 to 25th nucleotide of the transcript under standard conditions. The Fluorescein RNA Labeling Mix is specifically designed for use with the RNA polymerases SP6, T7, and T3, which are supplied with an optimized transcription buffer.
Preparation Note
10x concentrated NTP labeling mixture: 10mM ATP, 10mM CTP, 10mM GTP, 6.5mM UTP, 3.5mM Fluorescein-12-UTP, pH 7.5 (+20°C).
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
No data available
Flash Point(C)
No data available
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Zhimin Jiang et al.
Nature microbiology, 6(7), 932-945 (2021-05-15)
The retinoic acid-inducible gene I (RIG-I) receptor senses cytoplasmic viral RNA and activates type I interferons (IFN-I) and downstream antiviral immune responses. How RIG-I binds to viral RNA and how its activation is regulated remains unclear. Here, using IFI16 knockout
Lucia Caceres et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 248(12), 1243-1256 (2019-10-01)
Familial exudative vitreoretinopathy (FEVR) is a rare congenital disorder characterized by a lack of blood vessel growth to the periphery of the retina with secondary fibrovascular proliferation at the vascular-avascular junction. These structurally abnormal vessels cause leakage and hemorrhage, while
Anke J Roelofs et al.
Annals of the rheumatic diseases, 79(12), 1625-1634 (2020-09-24)
Osteophytes are highly prevalent in osteoarthritis (OA) and are associated with pain and functional disability. These pathological outgrowths of cartilage and bone typically form at the junction of articular cartilage, periosteum and synovium. The aim of this study was to
Yasufumi Hayano et al.
Science advances, 7(29) (2021-07-16)
The most prominent structural hallmark of the mammalian neocortical circuitry is the layer-based organization of specific cell types and synaptic inputs. Accordingly, cortical inhibitory interneurons (INs), which shape local network activity, exhibit subtype-specific laminar specificity of synaptic outputs. However, the
Paul A Dawson et al.
Biology of reproduction, 87(2), 43-43 (2012-06-08)
Sulfate is important for mammalian growth and development. During pregnancy, maternal circulating sulfate levels increase by 2-fold, enhancing sulfate availability to the fetus. We used quantitative real-time PCR to determine sulfate transporter mRNA levels during mouse gestation in three tissues:
Articles
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service