176901

Anthrax Lethal Factor Protease Inhibitor, In-2-LF

The Anthrax Lethal Factor Protease Inhibitor, In-2-LF controls the biological activity of Anthrax Lethal Factor Protease. This small molecule/inhibitor is primarily used for Protease Inhibitors applications.

• Synonym(s): Anthrax Lethal Factor Protease Inhibitor, In-2-LF, Anthrax LF Protease Inhibitor, Bacillus anthracis LF Protease Inhibitor, In-2-LF
• Empirical Formula (Hill Notation): C₈₁H₁₅₀N₄₂O₁₇
• Molecular Weight: 1984.33
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Quality Level: 100
• Assay: ≥97% (HPLC)
• form: lyophilized solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic)
• color: white
• solubility: water: 1 mg/mL; 5% acetic acid: 5 mg/mL; DMSO: 5 mg/mL
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

A cell-permeable N-acetylated, C-hydroxamate derivative of a 14-mer peptide designed from the MEK-2 template that acts as a competitive inhibitor of Anthrax lethal factor (LF) metalloprotease (K_i = 1 nM). Also inhibits MEK-3 cleavage. Protects against Anthrax toxin induced cytotoxicity in RAW264.7 and J772.A1 cells.

A cell-permeable derivative of a 14-mer peptide that is based on the MEK-2 template and acts as a competitive inhibitor of Anthrax lethal factor metalloprotease (K_i = 1 nM). Also inhibits MEK-3 cleavage. Exhibits protective effects against Anthrax toxin-induced cytotoxicity in RAW264.7 and J772.A1 cells. Reported to competitively inhibit furin (K_i = 49 nM.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
anthrax LF metalloprotease

Product does not compete with ATP.

Reversible: no

Target K_i: 1 nM against Anthrax lethal factor (LF) metalloprotease

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Sequence

Ac-Gly-Tyr-βAla-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Val-Leu-Arg-NHOH

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Peinado, J.R., et al. 2004. Biochem. Biophys. Res. Commun.321, 601.
Tonello, F., et. al. 2002. Nature418, 386.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable