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1.51456

Supelco

Purospher® STAR RP-18 endcapped (5 µm) HPLC Columns

L × I.D. 250 mm x 4.6 mm Hibar® RT HPLC Column

Synonym(s):

Purospher® STAR RP-18 endcapped (5 µm) Hibar® RT 250-4.6

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About This Item

UNSPSC Code:
41115709
NACRES:
SB.52
Pricing and availability is not currently available.

Product Name

Purospher® STAR RP-18 endcapped (5 µm), L × I.D. 250 mm x 4.6 mm Hibar® RT HPLC column

material

stainless steel column

Quality Level

100

Agency

suitable for USP L1

product line

Purospher®

feature

endcapped

packaging

1 ea of

extent of labeling

17% Carbon loading

parameter

400 bar pressure
65 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)

column L × I.D.

25 cm × 4.6 mm

surface area

330 m2/g

matrix

fully porous particle

matrix active group

C18 bonding phase

particle size

5 μm

pore size

120 Å pore size

operating pH range

1.5-10.5

separation technique

reversed phase
supercritical fluid (SFC)

storage temp.

no temp limit

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General description

Purospher® STAR RP-18 endcapped, 5 µm HPLC columns are designed for universal use and allow the separation of basic, neutral, and metal chelating compounds with simple mobile phases and excellent peak symmetry. They are based on ultra-pure fully porous silica particles (Type B). These columns offer an outstanding stability from pH 1.5 to 10.5 over a wide temperature range, and suitability for up to 100% aqueous mobile phases. Plus, they demonstrate best all-round retention characteristics, as proven by the Tanaka test. Thanks to these features, Purospher® STAR RP-18 endcapped, 5 µm HPLC columns allow maximum flexibility to use the best chromatographic conditions for any separation including reversed phase.Purospher® STAR RP-18 endcapped, 5 µm Hibar® RT 250-4.6 is a ready to use stainless steel column suitable for connection to any HPLC system.The column dimension of 250 mm length x 4.6 mm I.D. is the optimal choice for the high resolution HPLC separation of complex samples.The recommended guard column is Purospher® STAR RP-18 endcapped (5 µm) LiChroCART™ 4-4 part number 1.50250.0001. For direct connection to a Hibar® RT columns the Precolumn holder for 4-4 LiChroCART™ cartridges, part number 1.51487.0001 can be used.Item 1.51456.1003 is a Validation kit containing 3 Purospher® STAR RP-18 endcapped, 3 µm Hibar® RT 250-4.6 HPLC columns from 3 different base material lots.Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide

Application

Intended to be used in the separation of aflatoxins, aldehydes, alkaloids, antibiotics, catecholamines, explosives, oligonucleotides, esters, fat soluble vitamins, lipids, fatty acids, flavonoids, ketones, nitrosamines, nucleosides, PAHs, PCBs, peptides, pesticides, phenols, phthalates, preservatives, steroids, metabolized steroids, sulfonamides, sweeteners, and water soluble vitamins.

Features and Benefits

  • Ultra-pure silica (99.999%) for excellent peak symmetry
  • High separation efficiency
  • Reproducible results from run-to-run and batch-to-batch
  • Best all-round performance according to Tanaka
  • Outstanding pH stability from pH 1.5 to 10.5
  • No phase collapse when using highly aqueous mobile phases
  • Good suitability for LC-MS applications

Analysis Note

Pressure: ≤ 130 bar
Theoretical Plates (N/m) (Toluene): ≥ 80000
Symmetry (Toluene): 0.8 - 1.4
Capacity factor (Toluene): 1.0 - 3.0

Legal Information

Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany
LICHROCART is a trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Ephedrine/Pseudoephedrine HCl in Oral Solution Analysis

HPLC method on Purospher® RP-18e column analyzes ephedrine and pseudoephedrine HCl in traditional Chinese medicine.

Ephedrine/Pseudoephedrine HCl in Oral Solution Analysis

HPLC method on Purospher® RP-18e column analyzes ephedrine and pseudoephedrine HCl in traditional Chinese medicine.

Ephedrine/Pseudoephedrine HCl in Oral Solution Analysis

HPLC method on Purospher® RP-18e column analyzes ephedrine and pseudoephedrine HCl in traditional Chinese medicine.

Ephedrine/Pseudoephedrine HCl in Oral Solution Analysis

HPLC method on Purospher® RP-18e column analyzes ephedrine and pseudoephedrine HCl in traditional Chinese medicine.

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Protocols

Chromatographic Testing of Black Pepper

Powdered black pepper tested for pierine content follows USP guidelines using HPTLC and HPLC for dietary supplements.

Chromatographic Testing of Black Pepper

Powdered black pepper tested for pierine content follows USP guidelines using HPTLC and HPLC for dietary supplements.

Questions

1–2 of 2 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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