TBK, also known as IκB kinase (IKK)-related kinase NAK, belong to the serine/threonine IKK kinase family and is thought to play a role in NF-kappa-B (NF-κB) activation. The NF-κB complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NF-κB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NF-κB activation in response to certain growth factors. For example, the protein can form a complex with the IKB protein TANK and TRAF2 and release the NF-κB inhibition caused by TANK.
Specificity
This antibody recognizes TBK1 phosphorylated at Ser172.
Immunogen
KLH-conjugated linear peptide corresponding to human TBK1 phosphorylated at Ser172.
Application
This phospho-TBK1 antibody is validated for use in WB for the detection of the phospho-TBK1 protein.
Quality
Evaluated by Western Blot in EGF treated and untreated HEK293 cell lysates.
Western Blot Analysis: 1:1,000 dilution of this antibody detected TBK1 on 10 µg of EGF treated and untreated HEK293 cell lysates.
Target description
~90 kDa observed
Analysis Note
Control EGF treated and untreated HEK293 cell lysates
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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