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SRE0028

Sigma-Aldrich

Lipase from human pancreas

Synonym(s):

Lipase from human pancreas, Triacylglycerol acylhydrolase, Triacylglycerol lipase

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About This Item

Enzyme Commission number:
3.1.1.3 (BRENDA, IUBMB)
UNSPSC Code:
12352108
NACRES:
NA.54
Pricing and availability is not currently available.

form

lyophilized powder

Quality Level

100

storage temp.

−20°C

Related Categories

General description

Pancreatic lipase fulfills a key function in dietary fat absorption by hydrolyzing triglycerides into diglycerides, and subsequently into monoglycerides and free fatty acids.

Packaging

100 units in glass bottle

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SLCR6246
0000259597
SLCN9361
SLCQ0653
SLCN4141

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Questions

  1. Dear Sir/Madam, I purchased 100 Units of human lipase code SRE0028. How much buffer volume do I need to dissolve this lipase? If you can send me an instruction manual, I'd appreciate it. Kind regards,

    1 answer

    1. Unfortunately, there is no protocol provided for this product. This product should be reconstituted with Tris-buffered saline containing 1% BSA and 1 mM calcium chloride. For a similar, previously discontinued product, (L9780) there was a recommendation to reconstitute at 1 mg/mL for the best long term stability. Do not dilute to working concentration until ready to use. Solutions are stable for at least one month at 4 deg. C.

      See the link below to review the lipase enzymatic testing method:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/370/930/l0382enz.pdf

      Helpful?

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